ArticlePlant methods2014
Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies.
Article in Plant methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- A Fluorescence-Based Transient Expression Assay for the Analysis of Upstream Open Reading Frames in Plants.Plant direct · 2026Article
- Environmental dependence of visible anthocyanin accumulation mediated by theFrontiers in plant science · 2026Article
- A developmentally controlled cellular decompartmentalization process executes programmed cell death in the Arabidopsis root cap.The Plant cell · 2024Article
- RNA virus-mediated gene editing for tomato trait breeding.Horticulture research · 2024Article
- Repressive ZINC FINGER OF ARABIDOPSIS THALIANA proteins promote programmed cell death in the Arabidopsis columella root cap.Plant physiology · 2023Article
- Mining theFrontiers in plant science · 2023Article
- An Importin-β-like Protein fromViruses · 2021Article
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization.The Plant cell · 2021Article
- Biosensors: A Sneak Peek into Plant Cell's Immunity.Life (Basel, Switzerland) · 2021Review
- Comprehensive Review of Methodology to Detect Reactive Oxygen Species (ROS) in Mammalian Species and Establish Its Relationship with Antioxidants and Cancer.Antioxidants (Basel, Switzerland) · 2021Review
- Nanobody-Dependent Delocalization of Endocytic Machinery inFrontiers in plant science · 2021Article
- Overexpression of F-Box Nictaba Promotes Defense and Anthocyanin Accumulation inFrontiers in plant science · 2021Article
- A series of dual-reporter vectors for ratiometric analysis of protein abundance in plants.Plant direct · 2020Article
- CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis.The Plant cell · 2019Article
- Infectivity of cloned begomoviral DNAs: an appraisal.Virusdisease · 2019Article
- An atypical RNA silencing suppression strategy provides a snapshot of the evolution of sweet potato-infecting potyviruses.Scientific reports · 2018Article
- Co-expression with the Type 3 Secretion Chaperone CesT from EnterohemorrhagicFrontiers in plant science · 2017Article
- Reverse Genetics and High Throughput Sequencing Methodologies for Plant Functional Genomics.Current genomics · 2016Article
- Testing the utility of fluorescent proteins in Mimulus lewisii by an Agrobacterium-mediated transient assay.Plant cell reports · 2016Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent protein genes are among the most commonly used reporters in transient RNA silencing and heterologous protein expression assays. Fluorescence intensity is used to quantify fluorescent protein accumulation by image analysis or spectroscopy of protein extracts; however, these methods might not be suitable for medium- to large-scale comparisons.
resultsWe report that laser scanners, used routinely in proteomic studies, are suitable for quantitative imaging of plant leaves that express different fluorescent protein pairs. We developed a microtiter plate fluorescence spectroscopy method for direct quantitative comparison of fluorescent protein accumulation in intact leaf discs. We used this technique to measure a fluorescent reporter in a transient RNA silencing suppression assay, and also to monitor early amplification dynamics of a fluorescent protein-labeled potyvirus.
conclusionsLaser scanners allow dual-color fluorescence imaging of leaf samples, which might not be acquired in standard stereomicroscope devices. Fluorescence microtiter plate analysis of intact leaf discs can be used for rapid, accurate quantitative comparison of fluorescent protein accumulation.
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