ArticleMolecular vision2014
MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis.
Article in Molecular vision, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- Transcriptomics and network analysis highlight potential pathways in the pathogenesis of pterygium.Scientific reports · 2022Pooled it
- Co-host ncRNA MIR503HG/miR-503-5p antagonistically interfere with the crosstalk between fibroblasts and microvascular endothelial cells by affecting the production of LMW FGF2 in pterygium.International journal of ophthalmology · 2025Article
- Sequential expression of miR-221-3p and miR-338-3p in Schwann cells as a therapeutic strategy to promote nerve regeneration and functional recovery.Neural regeneration research · 2023Article
- MicroRNA and Their Potential Role in Conjunctival Disorders.Korean journal of ophthalmology : KJO · 2022Review
- Expression profiling suggests the involvement of hormone-related, metabolic, and Wnt signaling pathways in pterygium progression.Frontiers in endocrinology · 2022Article
- MiR-199a-3p/5p participated in TGF-β and EGF induced EMT by targeting DUSP5/MAP3K11 in pterygium.Journal of translational medicine · 2020Article
- Exploring the Molecular Mechanisms of Pterygium by Constructing lncRNA-miRNA-mRNA Regulatory Network.Investigative ophthalmology & visual science · 2020Article
- Identification of Functional Genes in Pterygium Based on Bioinformatics Analysis.BioMed research international · 2020Article
- Identification and Interaction Analysis of Significant Genes and MicroRNAs in Pterygium.BioMed research international · 2019Article
- Identification of pterygium-related mRNA expression profiling by microarray analysis.Eye (London, England) · 2017Article
- Expression of microRNAs in fibroblast of pterygium.International journal of ophthalmology · 2016Article
- Circulating microRNA-451 as a predictor of resistance to neoadjuvant chemotherapy in breast cancer.Cancer biomarkers : section A of Disease markers · 2016Article
- RNA-Sequencing Analysis of Messenger RNA/MicroRNA in a Rabbit Aneurysm Model Identifies Pathways and Genes of Interest.AJNR. American journal of neuroradiology · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeMiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium.
methodsWe collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation of pterygium cells.
resultsSeventy-two (60.0%) pterygial specimens showed high miR-221 expression levels, which was significantly higher than the control groups (13 of 120, 10.8%, p<0.0001). MiR-221 expression was significantly higher in β-catenin-nuclear/cytoplasmic-positive groups than in β-catenin membrane-positive and negative groups (p=0.001). We also found that p27Kip1 gene expression in pterygium was negatively correlated with miR-221 expression (p=0.002). In the clinical association, miR-221 expression was significantly higher in the fleshy and intermediate groups than in the atrophic group (p=0.007). The association of miR-221, p27Kip1 and proliferation of pterygium were also confirmed in the PECs model.
conclusionsOur study demonstrated that activation of β-catenin in pterygium may interact with miR-221, resulting in p27Kip1 gene downregulation that influences pterygium pathogenesis.
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Identifiers
25053875PMC4105113W1501414433What OpenQuestion holds
Registered trials
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