ArticleNucleic acids research2014
Is an observed non-co-linear RNA product spliced in trans, in cis or just in vitro?
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 64 citations in OpenAlex.
- Circular RNAs in Cardiovascular Physiopathology: From Molecular Mechanisms to Therapeutic Opportunities.International journal of molecular sciences · 2025Review
- Past, present, and future strategies for detecting and quantifying circular RNA variants.The FEBS journal · 2025Review
- Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma.Journal of microbiology and biotechnology · 2025Article
- Development of Mathematical Models Using circRNA Combinations (International journal of molecular sciences · 2025Article
- Viral circular RNA-encoded protein, ceVP28, divulges an antiviral response in invertebrates.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring the Post Mortem Interval (PMI) Estimation Model by circRNAInternational journal of molecular sciences · 2025Article
- Direct RNA sequencing in plants: Practical applications and future perspectives.Plant communications · 2024Review
- Long- and short-read sequencing methods discover distinct circular RNA pools in Lotus japonicus.The plant genome · 2024Article
- FL-circAS: an integrative resource and analysis for full-length sequences and alternative splicing of circular RNAs with nanopore sequencing.Nucleic acids research · 2024Article
- Article
- Detecting intragenic trans-splicing events from non-co-linearly spliced junctions by hybrid sequencing.Nucleic acids research · 2023Article
- Large-scale benchmarking of circRNA detection tools reveals large differences in sensitivity but not in precision.Nature methods · 2023Article
- Artifacts and biases of the reverse transcription reaction in RNA sequencing.RNA (New York, N.Y.) · 2023Review
- TargetedCell reports methods · 2023Article
- Assessing the impacts of various factors on circular RNA reliability.Life science alliance · 2023Article
- Recent advances in cancer fusion transcript detection.Briefings in bioinformatics · 2023Review
- Biogenesis and Regulatory Roles of Circular RNAs.Annual review of cell and developmental biology · 2022Review
- Best practice standards for circular RNA research.Nature methods · 2022Review
- Noncoding RNAs in apoptosis: identification and function.Turkish journal of biology = Turk biyoloji dergisi · 2022Review
- tsRNAs: Novel small molecules from cell function and regulatory mechanism to therapeutic targets.Cell proliferation · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Global transcriptome investigations often result in the detection of an enormous number of transcripts composed of non-co-linear sequence fragments. Such 'aberrant' transcript products may arise from post-transcriptional events or genetic rearrangements, or may otherwise be false positives (sequencing/alignment errors or in vitro artifacts). Moreover, post-transcriptionally non-co-linear ('PtNcl') transcripts can arise from trans-splicing or back-splicing in cis (to generate so-called 'circular RNA'). Here, we collected previously-predicted human non-co-linear RNA candidates, and designed a validation procedure integrating in silico filters with multiple experimental validation steps to examine their authenticity. We showed that >50% of the tested candidates were in vitro artifacts, even though some had been previously validated by RT-PCR. After excluding the possibility of genetic rearrangements, we distinguished between trans-spliced and circular RNAs, and confirmed that these two splicing forms can share the same non-co-linear junction. Importantly, the experimentally-confirmed PtNcl RNA events and their corresponding PtNcl splicing types (i.e. trans-splicing, circular RNA, or both sharing the same junction) were all expressed in rhesus macaque, and some were even expressed in mouse. Our study thus describes an essential procedure for confirming PtNcl transcripts, and provides further insight into the evolutionary role of PtNcl RNA events, opening up this important, but understudied, class of post-transcriptional events for comprehensive characterization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.