ArticleProceedings of the National Academy of Sciences of the United States of America2014
Stationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Mass-Activated Droplet Sorting for the Selection of Lysine-ProducingAnalytical chemistry · 2023Article
- Microfluidic static droplet generated quantum dot arrays as color conversion layers for full-color micro-LED displays.Nanoscale advances · 2023Article
- Modular microfluidics for life sciences.Journal of nanobiotechnology · 2023Review
- Surface Modification of 3D Printed Microfluidic Devices for Controlled Wetting in Two-Phase Flow.Micromachines · 2022Article
- Tracking the stochastic growth of bacterial populations in microfluidic droplets.Physical biology · 2022Article
- Single-cell sorting based on secreted products for functionally defined cell therapies.Microsystems & nanoengineering · 2022Review
- An easy-to-operate method for single-cell isolation and retrieval using a microfluidic static droplet array.Mikrochimica acta · 2021Article
- One cell at a time: droplet-based microbial cultivation, screening and sequencing.Marine life science & technology · 2021Review
- Single-Cell Phenotypic Analysis and Digital Molecular Detection Linkable by a Hydrogel Bead-Based Platform.ACS applied bio materials · 2021Article
- Microfluidic characterization of macromolecular liquid-liquid phase separation.Lab on a chip · 2020Article
- Capillary-Driven Flow Microfluidics Combined with Smartphone Detection: An Emerging Tool for Point-of-Care Diagnostics.Diagnostics (Basel, Switzerland) · 2020Review
- On-chip MIC by Combining Concentration Gradient Generator and Flanged Chamber Arrays.Micromachines · 2020Article
- DropSOAC: Stabilizing Microfluidic Drops for Time-Lapse Quantification of Single-Cell Bacterial Physiology.Frontiers in microbiology · 2019Article
- A power-free, parallel loading microfluidic reactor array for biochemical screening.Scientific reports · 2018Article
- Microfluidic chambers using fluid walls for cell biology.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography.Journal of visualized experiments : JoVE · 2018Article
- Gelatin Nanoparticle-Coated Silicon Beads for Density-Selective Capture and Release of Heterogeneous Circulating Tumor Cells with High Purity.Theranostics · 2018Article
- Multimodal microfluidic platform for controlled culture and analysis of unicellular organisms.Biomicrofluidics · 2017Article
- Rapid phenotypic antimicrobial susceptibility testing using nanoliter arrays.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Reducing Uncertainty for Acute Febrile Illness in Resource-Limited Settings: The Current Diagnostic Landscape.The American journal of tropical medicine and hygiene · 2017Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be applied for single-cell analysis; however, to investigate encapsulated cells effectively over prolonged time periods, an array of droplets must remain stationary on a versatile substrate for optimal cell compatibility. We present here a platform of unique geometry and substrate versatility that generates a stationary nanodroplet array by using wells branching off a main microfluidic channel. These droplets are confined by multiple sides of a nanowell and are in direct contact with a biocompatible substrate of choice. The device is operated by a unique and reversed loading procedure that eliminates the need for fine pressure control or external tubing. Fluorocarbon oil isolates the droplets and provides soluble oxygen for the cells. By using this approach, the metabolic activity of single adherent cells was monitored continuously over time, and the concentration of viable pathogens in blood-derived samples was determined directly by measuring the number of colony-formed droplets. The method is simple to operate, requires a few microliters of reagent volume, is portable, is reusable, and allows for cell retrieval. This technology may be particularly useful for multiplexed assays for which prolonged and simultaneous visual inspection of many isolated single adherent or nonadherent cells is required.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.