ArticleJournal of experimental botany2014
Engineering α-amylase levels in wheat grain suggests a highly sophisticated level of carbohydrate regulation during development.
Article in Journal of experimental botany, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Surface dielectric barrier discharge plasma priming enhances salt-tolerant wheat germination through predicted AMY1.1 redox stabilization.Scientific reports · 2026Article
- Balancing act: transcriptomic, morphological, and biochemical insights into wheat endosperm development associated with bread quality.BMC plant biology · 2026Article
- Molecular Characteristics and Functional Identification of a Key Alpha-Amylase-Encoding GeneInternational journal of molecular sciences · 2024Article
- GWAS in a Collection of Bulgarian Old and Modern Bread Wheat Accessions Uncovers Novel Genomic Loci for Grain Protein Content and Thousand Kernel Weight.Plants (Basel, Switzerland) · 2024Article
- Evaluation of altered starch mutants and identification of candidate genes responsible for starch variation in wheat.BMC plant biology · 2023Article
- Proteomic exploration reveals a metabolic rerouting due to low oxygen during controlled germination of malting barley (Frontiers in plant science · 2023Article
- A community resource to mass explore the wheat grain proteome and its application to the late-maturity alpha-amylase (LMA) problem.GigaScience · 2022Article
- Over-Expression of a Wheat Late Maturity Alpha-Amylase Type 1 Impact on Starch Properties During Grain Development and Germination.Frontiers in plant science · 2022Article
- Overexpression of a wheat α-amylase type 2 impact on starch metabolism and abscisic acid sensitivity during grain germination.The Plant journal : for cell and molecular biology · 2021Article
- Late-Maturity Alpha-Amylase in Wheat (Foods (Basel, Switzerland) · 2021Article
- Article
- Quantitative Trait Locus (QTLs) Mapping for Quality Traits of Wheat Based on High Density Genetic Map Combined With Bulked Segregant Analysis RNA-seq (BSR-Seq) Indicates That theFrontiers in plant science · 2020Article
- MaizeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- New insights into the origin and evolution of α-amylase genes in green plants.Scientific reports · 2019Article
- Structural organization and functional divergence of high isoelectric point α-amylase genes in bread wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.).BMC genetics · 2019Article
- Does Late Maturity Alpha-Amylase Impact Wheat Baking Quality?Frontiers in plant science · 2018Article
- Article
- Genetic architecture of kernel composition in global sorghum germplasm.BMC genomics · 2017Article
- Phosphorus Alters Starch Morphology and Gene Expression Related to Starch Biosynthesis and Degradation in Wheat Grain.Frontiers in plant science · 2017Article
- High Temperature-Induced Expression of Rice α-Amylases in Developing Endosperm Produces Chalky Grains.Frontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Wheat starch degradation requires the synergistic action of different amylolytic enzymes. Our spatio-temporal study of wheat α-amylases throughout grain development shows that AMY3 is the most abundant isoform compared with the other known α-amylases. Endosperm-specific over-expression of AMY3 resulted in an increase of total α-amylase activity in harvested grains. Unexpectedly, increased activity did not have a significant impact on starch content or composition but led to an increase of soluble carbohydrate (mainly sucrose) in dry grain. In AMY3 overexpression lines (A3OE), germination was slightly delayed and triacylglycerol (TAG) content was increased in the endosperm of mature grain. Despite increased AMY3 transcript and protein content throughout grain development, alterations of α-amylase activity and starch granule degradation were not detected until grain maturation, suggesting a post-translational inhibition of α-amylase activity in the endosperm during the starch filling period. These findings show unexpected effects of a high level of α-amylase on grain development and composition, notably in carbon partitioning and TAG accumulation, and suggest the presence of a hitherto unknown regulatory pathway during grain filling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.