ArticleDevelopment (Cambridge, England)2014
Single cell dissection of early kidney development: multilineage priming.
Article in Development (Cambridge, England), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
87 citing papers in PubMed.
- Mesenchymal WNT signaling coordinates epithelial and mesenchymal differentiation in the developing murine ureter.Cell communication and signaling : CCS · 2026Article
- The transcription factor Tcf21 is necessary for adoption of cell fates byAmerican journal of physiology. Renal physiology · 2026Article
- Activation-derepression synergy enables a bHLH network to coordinate a signal-specific fate response.Cell reports · 2024Article
- The replicative helicase CMG is required for the divergence of cell fates during asymmetric cell division in vivo.Nature communications · 2024Article
- AUTS2 disruption causes neuronal differentiation defects in human cerebral organoids through hyperactivation of the WNT/β-catenin pathway.Scientific reports · 2024Article
- Epithelial tubule interconnection driven by HGF-Met signaling in the kidney.bioRxiv : the preprint server for biology · 2024Article
- Fourteen years of cellular deconvolution: methodology, applications, technical evaluation and outstanding challenges.Nucleic acids research · 2024Review
- ETV2 primes hematoendothelial gene enhancers prior to hematoendothelial fate commitment.Cell reports · 2023Article
- Hedgehog-GLI mediated control of renal formation and malformation.Frontiers in nephrology · 2023Review
- Article
- Gene length is a pivotal feature to explain disparities in transcript capture between single transcriptome techniques.Frontiers in bioinformatics · 2023Article
- Characterization of alternative mRNA splicing in cultured cell populations representing progressive stages of human fetal kidney development.Scientific reports · 2022Article
- Omics profiling identifies the regulatory functions of the MAPK/ERK pathway in nephron progenitor metabolism.Development (Cambridge, England) · 2022Article
- Mapping the human kidney using single-cell genomics.Nature reviews. Nephrology · 2022Review
- Comparative whole-genome transcriptome analysis in renal cell populations reveals high tissue specificity of MAPK/ERK targets in embryonic kidney.BMC biology · 2022Article
- Patterning the embryonic pulmonary mesenchyme.iScience · 2022Article
- Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair.Science translational medicine · 2022Article
- Chromatin accessibility and microRNA expression in nephron progenitor cells during kidney development.Genomics · 2022Article
- Biological Sequence Classification: A Review on Data and General Methods.Research (Washington, D.C.) · 2022Article
- Single-cell transcriptomics: A new tool for studying diabetic kidney disease.Frontiers in physiology · 2022Review
27 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
We used a single cell RNA-seq strategy to create an atlas of gene expression patterns in the developing kidney. At several stages of kidney development, histologically uniform populations of cells give rise to multiple distinct lineages. We performed single cell RNA-seq analysis of total mouse kidneys at E11.5 and E12.5, as well as the renal vesicles at P4. We define an early stage of progenitor cell induction driven primarily by gene repression. Surprising stochastic expression of marker genes associated with differentiated cell types was observed in E11.5 progenitors. We provide a global view of the polarized gene expression already present in the renal vesicle, the first epithelial precursor of the nephron. We show that Hox gene read-through transcripts can be spliced to produce intergenic homeobox swaps. We also identify a surprising number of genes with partially degraded noncoding RNA. Perhaps most interesting, at early developmental times single cells often expressed genes related to several developmental pathways. This provides powerful evidence that initial organogenesis involves a process of multilineage priming. This is followed by a combination of gene repression, which turns off the genes associated with most possible lineages, and the activation of increasing numbers of genes driving the chosen developmental direction.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.