ArticleMedical microbiology and immunology2014
The role of CD4(+) T cells in BKV-specific T cell immunity.
Article in Medical microbiology and immunology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 43 citations in OpenAlex.
- SARS-CoV-2-specific T cell responses and correlations with COVID-19 patient predisposition.The Journal of clinical investigation · 2020Trial
- Review
- Urine-derived renal tubular epithelial cells resemble functional characteristics of professional antigen-presenting cells and can directly induce BKV-specific T cell responses.Journal of nephrology · 2025Article
- Live long and persist: polyomavirus immune evasion in the brain and kidney.Future virology · 2025Article
- Donor HLA-DQ genetic and functional divergence affect the control of BK polyoma virus infection after kidney transplantation.Science advances · 2025Article
- Suppression of large t antigen-stimulated CD4Frontiers in medicine · 2025Article
- Familial CD45RAFrontiers in medicine · 2023Article
- Association of donor hepatitis C virus infection status and risk of BK polyomavirus viremia after kidney transplantation.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2022Article
- The effect of BKV reactivation on cytokines behavior in kidney transplanted patients.BMC nephrology · 2022Article
- Detection of pre-existing SARS-CoV-2-reactive T cells in unexposed renal transplant patients.Journal of nephrology · 2021Article
- Robust hepatitis B vaccine-reactive T cell responses in failed humoral immunity.Molecular therapy. Methods & clinical development · 2021Article
- Immune monitoring facilitates the clinical decision in multifocal COVID-19 of a pancreas-kidney transplant patient.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2020Article
- BK Polyomavirus-specific T cell immune responses in kidney transplant recipients diagnosed with BK Polyomavirus-associated nephropathy.BMC infectious diseases · 2019Article
- BK Polyomavirus: Clinical Aspects, Immune Regulation, and Emerging Therapies.Clinical microbiology reviews · 2017Review
- BK nephropathy in the native kidneys of patients with organ transplants: Clinical spectrum of BK infection.World journal of transplantation · 2016Review
- Characterization of Immunodominant BK Polyomavirus 9mer Epitope T Cell Responses.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2016Article
- Early identification of renal transplant recipients with high risk of polyomavirus-associated nephropathy.Medical microbiology and immunology · 2015Article
- A revised strategy for monitoring BKV-specific cellular immunity in kidney transplant patients.Kidney international · 2015Article
- Increased frequency of JC-polyomavirus detection in rheumatoid arthritis patients treated with multiple biologics.Medical microbiology and immunology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactivation of polyomavirus BK (BKV) infection represents a severe complication in kidney transplant (KTX) patients. We previously reported an association between a declining BK viral load and the reconstitution of CD4(+) T cell BKV-specific immunity in patients following kidney transplantation. However, the specific contribution of CD4(+) T cells in the regulation of BKV-replication is unknown. Nevertheless, in vitro enrichment of BKV-specific T cells and subsequent adoptive T cell transfer may improve the restoration of immune competence in KTX patients with BKV infection. To date, strategies to capture human BKV-specific T cells with the ensuing expansion to clinically useful numbers are lacking. Here, we demonstrated a comprehensive flow cytometric analysis of the BKV-specific T cell response that permits access to the majority of T cells specific for immunodominant BKV antigens. A full-spectrum evaluation of the BKV-specific T cell response was performed by stimulating peripheral blood mononuclear cells (PBMC) with a mixture of BKV immunodominant peptide pools at varying concentrations and measuring activation marker expression and cytokine secretion. We also examined the effects of co-stimulation and PBMC resting time prior to activation. We defined the narrow range of stimulation conditions that permit the capture and expansion of functional BKV-specific T cell lines. The generated BKV-specific T cell lines showed the highest specificity and functionality when the T cells were captured according to IFNγ-secretion. This study highlights the multifunctional and cytolytic BKV-specific CD4(+) T cells as a dominant population within the generated T cell product. This method offers a novel approach for the generation of BKV-specific T cell lines for adoptive immunotherapy and underscores the critical role of CD4(+) T cells in the clearance of BKV.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.