ArticlePlant biotechnology journal2014
Expression of γ-tocopherol methyltransferase in chloroplasts results in massive proliferation of the inner envelope membrane and decreases susceptibility to salt and metal-induced oxidative stresses by reducing reactive oxygen species.
Article in Plant biotechnology journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 72 citations in OpenAlex.
- A century of vitamin E research: The innovative journey from basic biology to synthetic bio-manufacturing.Journal of integrative plant biology · 2026Review
- An alternative splicing caused by a natural variation in BnaC02.VTE4 gene affects vitamin E and glucosinolate content in rapeseed (Brassica napus L.).Plant biotechnology journal · 2025Article
- Vitamin E in Plants: Biosynthesis Pathways, Biofortification Strategies, and Regulatory Dynamics.International journal of molecular sciences · 2025Review
- Potential role of tocopherol in protecting crop plants against abiotic stresses.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Chloroplast Engineering: Fundamental Insights and Its Application in Amelioration of Environmental Stress.Applied biochemistry and biotechnology · 2023Review
- Review
- Nutritional Enrichment of Plant Leaves by Combining Genes Promoting Tocopherol Biosynthesis and Storage.Metabolites · 2023Article
- Photosynthetic machinery under salinity stress: Trepidations and adaptive mechanisms.Photosynthetica · 2023Review
- Effect of Overexpression ofInternational journal of molecular sciences · 2022Article
- Defining key metabolic roles in osmotic adjustment and ROS homeostasis in the recretohalophyte Karelinia caspia under salt stress.Physiologia plantarum · 2022Article
- Insights into plastome ofSaudi journal of biological sciences · 2022Article
- Vitamin E synthesis and response in plants.Frontiers in plant science · 2022Review
- ROS scavenging and ion homeostasis is required for the adaptation of halophyteFrontiers in plant science · 2022Article
- Molecular Mechanisms of Plant Responses to Salt Stress.Frontiers in plant science · 2022Review
- Green giant-a tiny chloroplast genome with mighty power to produce high-value proteins: history and phylogeny.Plant biotechnology journal · 2021Review
- Article
- The Genome and Transcriptome Analysis of thePlants (Basel, Switzerland) · 2020Article
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Study of subcellular localization of3 Biotech · 2020Article
- Comparative analysis of cp genome ofSaudi journal of biological sciences · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The γ-tocopherol methyltransferase (γ-TMT) is an important enzyme regulating synthesis of four tocopherols (α, γ, β and δ). In this report, we investigated the role of γ-TMT in regulating abiotic stress within chloroplasts. The At γ-tmt overexpressed via the tobacco chloroplast genome accumulated up to 7.7% of the total leaf protein, resulting in massive proliferation of the inner envelope membrane (IEM, up to eight layers). Such high-level expression of γ-TMT converted most of γ-tocopherol to α-tocopherol in transplastomic seeds (~10-fold higher) in the absence of abiotic stress. When grown in 400 mm NaCl, α-tocopherol content in transplastomic TMT leaves increased up to 8.2-fold and 2.4-fold higher than wild-type leaves. Likewise, under heavy metal stress, α-tocopherol content in the TMT leaves increased up to 7.5-fold, twice higher than in the wild type. Under extreme salt stress, the wild type accumulated higher starch and total soluble sugars, but TMT plants were able to regulate sugar transport. Hydrogen peroxide and superoxide content in wild type increased up to 3-fold within 48 h of NaCl stress when compared to TMT plants. The ion leakage from TMT leaves was significantly less than wild-type plants under abiotic stress and with less malondialdehyde, indicating lower lipid peroxidation. Taken together, these studies show that α-tocopherol plays a crucial role in the alleviation of salt and heavy metal stresses by decreasing ROS, lipid peroxidation and ion leakage, in addition to enhancing vitamin E conversion. Increased proliferation of the IEM should facilitate studies on retrograde signalling from chloroplast to the nucleus.
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Registered trials
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