Evidence map›Paper›PMID 25051506›Full record

ArticleMolecular medicine reports2014

Combined effect of nerve growth factor and brain‑derived neurotrophic factor on neuronal differentiation of neural stem cells and the potential molecular mechanisms.

Feifei Liu, Aiguo Xuan, Yan Chen, Jundu Zhang, Liping Xu, Qijiang Yan, Dahong Long

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
42citing papers in PubMed, 1 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

42 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.

  1. Pooled it
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  12. MASH1 induces neuron transdifferentiation of adrenal medulla chromaffin cells.Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2023
    Article
  13. Differential contribution of TrkB and p75Frontiers in molecular neuroscience · 2023
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Feifei LiuDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Aiguo XuanDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Yan ChenDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Jundu ZhangDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Liping XuDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Qijiang YanKingmed Diagnostics College, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Dahong LongDepartment of Human Anatomy, Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China.
Guangzhou Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Neural stem cells (NSCs) are important pluripotent stem cells, which have potential applications in cell replacement therapy. Brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) have been demonstrated to exert a marked impact on the proliferation and differentiation of NSCs. The effects of NGF, BDNF, and BDNF combined with NGF on NSC neuronal differentiation and the possible mechanisms for these effects were investigated in this study. An adherent monolayer culture was employed to obtain highly homogeneous NSCs. The cells were divided into four groups: Control, NGF, BDNF and combination (BDNF + NGF) groups. Neuron differentiation was examined using immunocytochemistry and phospho-extracellular signal-regulated kinase (p‑ERK) levels were analyzed using western blotting. Reverse transcription polymerase chain reaction was used to measure the mRNA expression levels of the HES1, HES5, MASH1, NGN1 and NeuroD transcription factors at different time intervals following neurotrophin-induced differentiation. NGF and BDNF were observed to induce NSC neuronal differentiation, and β-tubulin III-positive cells and p-ERK expression levels were highest in the NGF + BDNF combination group at all time points. The proportion of β-tubulin Ⅲ-positive neurons in each group was associated with the expression levels of MASH1, NGN1 and NeuroD in the group. In conclusion, BDNF combined with NGF significantly improved NSC neuronal differentiation, which may provide support for the practical application of NSCs in neurodegenerative diseases.

Indexed as

AnimalsBasic Helix-Loop-Helix ProteinsBrain-Derived Neurotrophic FactorCell DifferentiationCells, CulturedEmbryo, MammalianFemaleFlavonoidsMAP Kinase Signaling SystemMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Nerve Growth FactorsNerve Tissue ProteinsNeural Stem CellsRatsRats, Sprague-Dawley2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneAscl1 protein, ratBasic Helix-Loop-Helix ProteinsBrain-Derived Neurotrophic FactorFlavonoidsMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Nerve Growth FactorsNerve Tissue ProteinsNeurogenic differentiation factor 1neurogenin-1, ratRNA, MessengerTubulin

Identifiers

PMID25051506
PMCPMC4148384
OpenAlexW2004504096

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.