ArticleEndocrinology2014
Ezrin is an actin binding protein that regulates sertoli cell and spermatid adhesion during spermatogenesis.
Article in Endocrinology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Gonadotropins differentially regulate testicular cell adhesion and junctional complexes during flatfish spermiogenesis through the oxytocin and relaxin signaling pathways.Frontiers in cell and developmental biology · 2025Article
- Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis.Development (Cambridge, England) · 2023Article
- Epidermal growth factor receptor signaling uncouples germ cells from the somatic follicular compartment at ovulation.Nature communications · 2021Article
- Prediction of 3D Protein Structure Based on The Mutation ofInternational journal of fertility & sterility · 2020Article
- Article
- FoxO transcription factors 1 regulate mouse preimplantation embryo development.Journal of assisted reproduction and genetics · 2019Article
- Ezrin Promotes Stem Cell Properties in Pancreatic Ductal Adenocarcinoma.Molecular cancer research : MCR · 2019Article
- Properties, metabolism and roles of sulfogalactosylglycerolipid in male reproduction.Progress in lipid research · 2018Review
- Drebrin and Spermatogenesis.Advances in experimental medicine and biology · 2017Article
- Cell polarity proteins and spermatogenesis.Seminars in cell & developmental biology · 2016Review
- Is toxicant-induced Sertoli cell injury in vitro a useful model to study molecular mechanisms in spermatogenesis?Seminars in cell & developmental biology · 2016Review
- From the Cover: Sperm Molecular Biomarkers Are Sensitive Indicators of Testicular Injury following Subchronic Model Toxicant Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Polarity protein Crumbs homolog-3 (CRB3) regulates ectoplasmic specialization dynamics through its action on F-actin organization in Sertoli cells.Scientific reports · 2016Article
- Coordination of Actin- and Microtubule-Based Cytoskeletons Supports Transport of Spermatids and Residual Bodies/Phagosomes During Spermatogenesis in the Rat Testis.Endocrinology · 2016Article
- Regulation of blood-testis barrier by actin binding proteins and protein kinases.Reproduction (Cambridge, England) · 2016Review
- GAR22β regulates cell migration, sperm motility, and axoneme structure.Molecular biology of the cell · 2016Article
- Effective Delivery of Male Contraceptives Behind the Blood-Testis Barrier (BTB) - Lesson from Adjudin.Current medicinal chemistry · 2016Review
- Actin binding proteins in blood-testis barrier function.Current opinion in endocrinology, diabetes, and obesity · 2015Review
- Merlin Isoforms 1 and 2 Both Act as Tumour Suppressors and Are Required for Optimal Sperm Maturation.PloS one · 2015Article
- Plastins regulate ectoplasmic specialization via its actin bundling activity on microfilaments in the rat testis.Asian journal of andrologyReview
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
During spermatogenesis, the transport of spermatids and the release of sperms at spermiation and the remodeling of the blood-testis barrier (BTB) in the seminiferous epithelium of rat testes require rapid reorganization of the actin-based cytoskeleton. However, the mechanism(s) and the regulatory molecule(s) remain unexplored. Herein we report findings that unfold the functional significance of ezrin in the organization of the testis-specific adherens junction at the spermatid-Sertoli cell interface called apical ectoplasmic specialization (ES) in the adluminal compartment and the Sertoli cell-cell interface known as basal ES at the BTB. Ezrin is expressed at the basal ES/BTB in all stages, except from late VIII to IX, of the epithelial cycle. Its knockdown by RNA interference (RNAi) in vitro perturbs the Sertoli cell tight junction-permeability barrier via a disruption of the actin microfilaments in Sertoli cells, which in turn impeded basal ES protein (eg, N-cadherin) distribution, perturbing the BTB function. These findings were confirmed by a knockdown study in vivo. However, the expression of ezrin at the apical ES is restricted to stage VIII of the cycle and limited only between step 19 spermatids and Sertoli cells. A knockdown of ezrin in vivo by RNAi was found to impede spermatid transport, causing defects in spermiation in which spermatids were embedded deep inside the epithelium, and associated with a loss of spermatid polarity. Also, ezrin was associated with residual bodies and phagosomes, and its knockdown by RNAi in the testis also impeded the transport of residual bodies/phagosomes from the apical to the basal compartment. In summary, ezrin is involved in regulating actin microfilament organization at the ES in rat testes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.