ArticleBritish journal of cancer2014
Unique volatolomic signatures of TP53 and KRAS in lung cells.
Article in British journal of cancer, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Diagnosis and Classification of 17 Diseases from 1404 Subjects via Pattern Analysis of Exhaled Molecules.ACS nano · 2017Pooled it
- Metabolomics and Biomarker Discovery.Advances in experimental medicine and biology · 2026Review
- Controlling glycolysis to generate characteristic volatile organic compounds of lung cancer cells.Scientific reports · 2024Article
- Partial role of volatile organic compounds in behavioural responses of mice to bedding from cancer-affected congeners.Biology open · 2024Article
- Nematode-Applied Technology for Human Tumor Microenvironment Research and Development.Current issues in molecular biology · 2022Review
- Volatolomics in healthcare and its advanced detection technology.Nano research · 2022Review
- Profiling Single Cancer Cells with Volatolomics Approach.iScience · 2019Article
- Article
- Discrimination between the human prostate normal and cancer cell exometabolome by GC-MS.Scientific reports · 2018Article
- Volatile fingerprinting of human respiratory viruses from cell culture.Journal of breath research · 2018Article
- The 150 most important questions in cancer research and clinical oncology series: questions 67-75 : Edited by Chinese Journal of Cancer.Chinese journal of cancer · 2017Article
- Volatile metabolomic signature of human breast cancer cell lines.Scientific reports · 2017Article
- The Pursuit of Noninvasive Diagnosis of Lung Cancer.Seminars in respiratory and critical care medicine · 2016Review
- Significance of Exhaled Breath Test in Clinical Diagnosis: A Special Focus on the Detection of Diabetes Mellitus.Journal of medical and biological engineering · 2016Review
- Article
- Investigation of VOCs associated with different characteristics of breast cancer cells.Scientific reports · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVolatile organic compounds (VOCs) are potential biomarkers for cancer detection in breath, but it is unclear if they reflect specific mutations. To test this, we have compared human bronchial epithelial cell (HBEC) cell lines carrying the KRAS(V12) mutation, knockdown of TP53 or both with parental HBEC cells.
methodsVOC from headspace above cultured cells were collected by passive sampling and analysed by thermal desorption gas chromatography mass spectrometry (TD-GC-MS) or sensor array with discriminant factor analysis (DFA).
resultsIn TD-GC-MS analysis, individual compounds had limited ability to discriminate between cell lines, but by applying DFA analysis combinations of 20 VOCs successfully discriminated between all cell types (accuracies 80-100%, with leave-one-out cross validation). Sensor array detection DFA demonstrated the ability to discriminate samples based on their cell type for all comparisons with accuracies varying between 77% and 93%.
conclusionsOur results demonstrate that minimal genetic changes in bronchial airway cells lead to detectable differences in levels of specific VOCs identified by TD-GC-MS or of patterns of VOCs identified by sensor array output. From the clinical aspect, these results suggest the possibility of breath analysis for detection of minimal genetic changes for earlier diagnosis or for genetic typing of lung cancers.
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