Evidence map›Paper›PMID 25050490›Full record

ArticleInternational journal of molecular medicine2014

Cold atmospheric plasma treatment selectively targets head and neck squamous cell carcinoma cells.

Rafael Guerrero-Preston, Takenori Ogawa, Mamoru Uemura, Gary Shumulinsky, Blanca L Valle, Francesca Pirini, Rajani Ravi, David Sidransky, Michael Keidar, Barry Trink

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
102citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

102 citing papers in PubMed, 3 syntheses or guidelines pooled it.

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42 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rafael Guerrero-PrestonDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Takenori OgawaDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Mamoru UemuraDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Gary ShumulinskyDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Blanca L ValleDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Francesca PiriniDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Rajani RaviDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
David SidranskyDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Michael KeidarDepartment of Mechanical and Aerospace Engineering, School of Engineering and Applied Science, The George Washington University, Washington, DC, USA.
Barry TrinkDepartment of Otolaryngology, Division of Head and Neck Cancer Research, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.

Funding

INTEGRATED DEVELOPMENT OF NOVEL MOLECULAR MARKERSU01CA084986 · NCI · JOHNS HOPKINS UNIVERSITY · PI SIDRANSKY, DAVID · 1999 to 2014
$33.8M
Epigenomic Markers of HNSCC Survival Across Ethnic GroupsK01CA164092 · NCI · JOHNS HOPKINS UNIVERSITY · PI GUERRERO-PRESTON, RAFAEL · 2011 to 2014
$530k
NCI NIH HHS K01 CA164092NCI NIH HHS K01CA164092NCI NIH HHS U01 CA084986NCI NIH HHS U01CA84986
6 · The paper itself

Abstract

The treatment of locoregional recurrence (LRR) of head and neck squamous cell carcinoma (HNSCC) often requires a combination of surgery, radiation therapy and/or chemotherapy. Survival outcomes are poor and the treatment outcomes are morbid. Cold atmospheric plasma (CAP) is an ionized gas produced at room temperature under laboratory conditions. We have previously demonstrated that treatment with a CAP jet device selectively targets cancer cells using in vitro melanoma and in vivo bladder cancer models. In the present study, we wished to examine CAP selectivity in HNSCC in vitro models, and to explore its potential for use as a minimally invasive surgical approach that allows for specific cancer cell or tumor tissue ablation without affecting the surrounding healthy cells and tissues. Four HNSCC cell lines (JHU-022, JHU-028, JHU-029, SCC25) and 2 normal oral cavity epithelial cell lines (OKF6 and NOKsi) were subjected to cold plasma treatment for durations of 10, 30 and 45 sec, and a helium flow of 20 l/min-1 for 10 sec was used as a positive treatment control. We showed that cold plasma selectively diminished HNSCC cell viability in a dose-response manner, as evidenced by MTT assays; the viability of the OKF6 cells was not affected by the cold plasma. The results of colony formation assays also revealed a cell-specific response to cold plasma application. Western blot analysis did not provide evidence that the cleavage of PARP occurred following cold plasma treatment. In conclusion, our results suggest that cold plasma application selectively impairs HNSCC cell lines through non-apoptotic mechanisms, while having a minimal effect on normal oral cavity epithelial cell lines.

Indexed as

AtmosphereBlotting, WesternCarcinoma, Squamous CellCell Line, TumorCell SurvivalHead and Neck NeoplasmsHumansPlasma GasesPoly(ADP-ribose) PolymerasesSquamous Cell Carcinoma of Head and NeckTumor Stem Cell AssayPlasma GasesPoly(ADP-ribose) Polymerases

Identifiers

PMID25050490
PMCPMC4152136

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.