ArticleProtein expression and purification2014
A scalable method for biochemical purification of Salmonella flagellin.
Article in Protein expression and purification, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 47 citations in OpenAlex.
- Safety and Immunogenicity of a Trivalent Salmonella Conjugate Vaccine to S. Typhi, S. Typhimurium, and S. Enteritidis.The Journal of infectious diseases · 2026Trial
- A combination typhoid and non-typhoidal Salmonella polysaccharide conjugate vaccine in healthy adults: a randomized, placebo-controlled phase 1 trial.Nature medicine · 2025Trial
- A phase 1 randomized, placebo-controlled trial of a combination typhoid and non-typhoidalmedRxiv : the preprint server for health sciences · 2025Article
- Advancing foodborne pathogen detection: a review of traditional and innovative optical and electrochemical biosensing approaches.Mikrochimica acta · 2025Review
- A simple cost-effective method for purification ofVeterinary research forum : an international quarterly journal · 2025Article
- Genetic engineering ofEcoSal Plus · 2024Review
- Bonded straight and helical flagellar filaments form ultra-low-density glasses.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Complementary measurement of nontyphoidalHeliyon · 2023Article
- Advances in the development of Salmonella-based vaccine strategies for protection against Salmonellosis in humans.Journal of applied microbiology · 2021Review
- MUC1 ectodomain is a flagellin-targeting decoy receptor and biomarker operative during Pseudomonas aeruginosa lung infection.Scientific reports · 2021Article
- Two homologous Salmonella serogroup C1-specific genes are required for flagellar motility and cell invasion.BMC genomics · 2021Article
- Overview of the Nontyphoidal and Paratyphoidal Salmonella Vaccine Pipeline: Current Status and Future Prospects.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2020Review
- Neuraminidase 1-mediated desialylation of the mucin 1 ectodomain releases a decoy receptor that protects againstThe Journal of biological chemistry · 2019Article
- Immunogenicity and protective efficacy againstHuman vaccines & immunotherapeutics · 2019Article
- Maternal Antibodies Elicited by Immunization With an O- Polysaccharide Glycoconjugate Vaccine Protect Infant Mice Against LethalFrontiers in immunology · 2019Article
- Deletions inHuman vaccines & immunotherapeutics · 2019Article
- Immunogenicity and Induction of Functional Antibodies in Rabbits Immunized with a Trivalent Typhoid-Invasive NontyphoidalMolecules (Basel, Switzerland) · 2018Article
- Immunogenicity and efficacy following sequential parenterally-administered doses of Salmonella Enteritidis COPS:FliC glycoconjugates in infant and adult mice.PLoS neglected tropical diseases · 2018Article
- Construction and evaluation of type III secretion system mutants of the catfish pathogen Edwardsiella piscicida.Journal of fish diseases · 2018Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Flagellins are the main structural proteins of bacterial flagella and potent stimulators of innate and adaptive immunity in mammals. The flagellins of Salmonella are virulence factors and protective antigens, and form the basis of promising vaccines. Despite broad interest in flagellins as antigens and adjuvants in vaccine formulations, there have been few advances towards the development of scalable and economical purification methods for these proteins. We report here a simple and robust strategy to purify flagellin monomers from the supernatants of liquid growth culture. Phase 1 flagellins from Salmonella enterica serovars Typhimurium (i epitope) and Enteritidis (g,m epitopes) were purified directly from conditioned fermentation growth media using sequential cation- and anion-exchange chromatography coupled with a final tangential flow-filtration step. Conventional porous chromatography resin was markedly less efficient than membrane chromatography for flagellin purification. Recovery after each process step was robust, with endotoxin, nucleic acid and residual host-cell protein effectively removed. The final yield was 200-300 mg/L fermentation culture supernatant, with ∼45-50% overall recovery. A final pH 2 treatment step was instituted to ensure uniformity of flagellin in the monomeric form. Flagellins purified by this method were recognized by monoclonal anti-flagellin antibodies and maintained capacity to activate Toll-like Receptor 5. The process described is simple, readily scalable, uses standard bioprocess methods, and requires only a few steps to obtain highly purified material.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.