ArticlePhysiology and molecular biology of plants : an international journal of functional plant biology2014
A reliable and efficient protocol for inducing genetically transformed roots in medicinal plant Nepeta pogonosperma.
Article in Physiology and molecular biology of plants : an international journal of functional plant biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 33 citations in OpenAlex.
- The hepato- and neuroprotective effect of gold Casuarina equisetifolia bark nano-extract against Chlorpyrifos-induced toxicity in rats.Journal, genetic engineering & biotechnology · 2023Article
- Effect of silver nanoparticles on tropane alkaloid production of transgenic hairy root cultures of Hyoscyamus muticus L. and their antimicrobial activity.Scientific reports · 2023Article
- Comparison of antibacterial and antioxidant potentials of pure and nanoemulsifiedFood science & nutrition · 2023Article
- Expression and mutagenesis studies in theFrontiers in plant science · 2023Article
- Enhanced inulin production by hairy root cultures ofMolecular biology research communications · 2021Article
- Highly efficient method ofPlant biotechnology (Tokyo, Japan) · 2020Article
- Article
- Influence of nano-zinc oxide on tropane alkaloid production,Engineering in life sciences · 2019Article
- Influence of different3 Biotech · 2018Article
- In vitro regeneration and Agrobacterium mediated genetic transformation of Artemisia aucheri Boiss.Physiology and molecular biology of plants : an international journal of functional plant biology · 2014Article
- Enhanced production of hyoscyamine and scopolamine from genetically transformed root culture ofIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Nepeta pogonosperma is an important medicinal plant with anti-inflammatory effects. An efficient and reliable transformation system for this plant was developed through optimization of several factors which affected the rate of Agrobacterium rhizogenes mediated transformation. Five bacterial strains, A4, ATCC15834, LBA9402, MSU440 and A13, two explant types, leaves and stems, and several co-cultivation media were examined. The maximum rate of hairy root induction was obtained from stem explants using MSU440 and ATCC15834 bacterial strains. A drastic increase in the frequency of transformation (91 %) was observed when MS medium lacking NH4NO3, KH2PO4, KNO3 and CaCl2. Hairy root lines were confirmed by polymerase chain reaction (PCR) using primers of the rolB gene. According to Southern blot analysis, one T-DNA copy was inserted into each of the hairy root lines. In the present study, transgenic hairy roots have been obtained trough genetic transformation by A. rhizogenes harbouring two plasmids, the Ri plasmid and pBI121 binary vector harbouring gus reporter gene. Expression of the gus gene in transgenic hairy root was confirmed by histochemical GUS assay.
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