ArticleProceedings of the National Academy of Sciences of the United States of America2014
Auxin transport sites are visualized in planta using fluorescent auxin analogs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 85 citations in OpenAlex.
- Short-day photoperiod enhances graft success by increasing auxin accumulation.Plant signaling & behavior · 2026Article
- Illuminating the subcellular maze: fluorescence-activated organelle sorting in plant sciences.Journal of experimental botany · 2025Review
- Cytosolic- and membrane-localized oxidized indole-3-acetic acid formation regulates developmental auxin transients.Plant physiology · 2025Article
- A century of studying plant secondary metabolism-From "what?" to "where, how, and why?"Plant physiology · 2024Review
- A novel toolbox to record CLE peptide signaling.Frontiers in plant science · 2024Article
- Changes in cell wall composition due to a pectin biosynthesis enzyme GAUT10 impact root growth.Plant physiology · 2023Article
- Encapsulation Reduces the Deleterious Effects of Salicylic Acid Treatments on Root Growth and Gravitropic Response.International journal of molecular sciences · 2022Article
- New Wine in an Old Bottle: Utilizing Chemical Genetics to Dissect Apical Hook Development.Life (Basel, Switzerland) · 2022Review
- Fluorescent Auxin Analogs Report Two Auxin Binding Sites with Different Subcellular Distribution and Affinities: A Cue for Non-Transcriptional Auxin Signaling.International journal of molecular sciences · 2022Article
- Auxin Transporters-A Biochemical View.Cold Spring Harbor perspectives in biology · 2022Review
- Fluorescent biosensors illuminating plant hormone research.Plant physiology · 2021Review
- Bioprospecting Fluorescent Plant Growth Regulators from Arabidopsis to Vegetable Crops.International journal of molecular sciences · 2021Article
- Sensors for the quantification, localization and analysis of the dynamics of plant hormones.The Plant journal : for cell and molecular biology · 2021Review
- Auxin-induced actin cytoskeleton rearrangements require AUX1.The New phytologist · 2020Article
- Direct comparison of Arabidopsis gene expression reveals different responses to melatonin versus auxin.BMC plant biology · 2019Article
- Auxin transport and stem vascular reconnection - has our thinking become canalized?Annals of botany · 2019Review
- Accumulation of and Response to Auxins in Roots and Nodules of the Actinorhizal PlantFrontiers in plant science · 2019Article
- Advances in research on the carrot, an important root vegetable in the Apiaceae family.Horticulture research · 2019Review
- Auxins and Cytokinins-The Role of Subcellular Organization on Homeostasis.International journal of molecular sciences · 2018Review
- What Has Been Seen Cannot Be Unseen-Detecting Auxin In Vivo.International journal of molecular sciences · 2017Review
Corrections and comments
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Authors and funding
10 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The plant hormone auxin is a key morphogenetic signal that controls many aspects of plant growth and development. Cellular auxin levels are coordinately regulated by multiple processes, including auxin biosynthesis and the polar transport and metabolic pathways. The auxin concentration gradient determines plant organ positioning and growth responses to environmental cues. Auxin transport systems play crucial roles in the spatiotemporal regulation of the auxin gradient. This auxin gradient has been analyzed using SCF-type E3 ubiquitin-ligase complex-based auxin biosensors in synthetic auxin-responsive reporter lines. However, the contributions of auxin biosynthesis and metabolism to the auxin gradient have been largely elusive. Additionally, the available information on subcellular auxin localization is still limited. Here we designed fluorescently labeled auxin analogs that remain active for auxin transport but are inactive for auxin signaling and metabolism. Fluorescent auxin analogs enable the selective visualization of the distribution of auxin by the auxin transport system. Together with auxin biosynthesis inhibitors and an auxin biosensor, these analogs indicated a substantial contribution of local auxin biosynthesis to the formation of auxin maxima at the root apex. Moreover, fluorescent auxin analogs mainly localized to the endoplasmic reticulum in cultured cells and roots, implying the presence of a subcellular auxin gradient in the cells. Our work not only provides a useful tool for the plant chemical biology field but also demonstrates a new strategy for imaging the distribution of small-molecule hormones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.