ArticlePlant physiology2014
bHLH05 is an interaction partner of MYB51 and a novel regulator of glucosinolate biosynthesis in Arabidopsis.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 125 citations in OpenAlex.
- BoOFP6 Negatively Regulates Glucosinolates Accumulation in Broccoli Sprouts by Cytoplasmic Sequestration of BoMYB34-3.Plant, cell & environment · 2026Article
- Microbial elicitors to metabolic reprogramming: an integrative model of plant-microbe interactions.Frontiers in microbiology · 2026Review
- Long-term methyl jasmonate exposure triggers COI1-independent induction of myrosinasesFrontiers in plant science · 2026Article
- Arabidopsis MYB47 and MYB95 transcription factors regulate jasmonate-inducible ER-body formation.Communications biology · 2025Article
- CsMYB213-CsbHLH35 module regulates theanine biosynthesis in tea plants (Camellia sinensis L.).Plant biotechnology journal · 2025Article
- Distribution of glucosinolates and antioxidants in different leaf-layers of Kale (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Deciphering the heterogeneous glucosinolates composition in leaves and seeds: strategies for developing Brassica napus genotypes with low seed glucosinolates content but high leaf glucosinolates content.Molecular horticulture · 2025Article
- Analysis of the quadruple lsu mutant reveals molecular determinants of the role of LSU proteins in sulfur assimilation in Arabidopsis.The Plant journal : for cell and molecular biology · 2024Article
- Chromosome-scale reference genome of broccoli (Horticulture research · 2024Article
- Sulfate Availability and Hormonal Signaling in the Coordination of Plant Growth and Development.International journal of molecular sciences · 2024Review
- Evolution and comparative transcriptome analysis of glucosinolate pathway genes inFrontiers in plant science · 2024Article
- New insights into the regulation of plant metabolism by O-acetylserine: sulfate and beyond.Journal of experimental botany · 2023Article
- A Revised View of theInternational journal of molecular sciences · 2023Review
- Regulation of the regulators: Transcription factors controlling biosynthesis of plant secondary metabolites during biotic stresses and their regulation by miRNAs.Frontiers in plant science · 2023Review
- Root and shoot biology of Arabidopsis halleri dissected by WGCNA: an insight into the organ pivotal pathways and genes of an hyperaccumulator.Functional & integrative genomics · 2022Article
- Structural insights into partner selection for MYB and bHLH transcription factor complexes.Nature plants · 2022Article
- Preharvest Methyl Jasmonate Treatment Increased Glucosinolate Biosynthesis, Sulforaphane Accumulation, and Antioxidant Activity of Broccoli.Antioxidants (Basel, Switzerland) · 2022Article
- Effects of Plant Hormones, Metal Ions, Salinity, Sugar, and Chemicals Pollution on Glucosinolate Biosynthesis in Cruciferous Plant.Frontiers in plant science · 2022Review
- Genome-wide identification of the Capsicum bHLH transcription factor family: discovery of a candidate regulator involved in the regulation of species-specific bioactive metabolites.BMC plant biology · 2021Article
- Evolution of A bHLH Interaction Motif.International journal of molecular sciences · 2021Article
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By means of yeast (Saccharomyces cerevisiae) two-hybrid screening, we identified basic helix-loop-helix transcription factor05 (bHLH05) as an interacting partner of MYB51, the key regulator of indolic glucosinolates (GSLs) in Arabidopsis (Arabidopsis thaliana). Furthermore, we show that bHLH04, bHLH05, and bHLH06/MYC2 also interact with other R2R3-MYBs regulating GSL biosynthesis. Analysis of bhlh loss-of-function mutants revealed that the single bhlh mutants retained GSL levels that were similar to those in wild-type plants, whereas the triple bhlh04/05/06 mutant was depleted in the production of GSL. Unlike bhlh04/06 and bhlh05/06 mutants, the double bhlh04/05 mutant was strongly affected in the production of GSL, pointing to a special role of bHLH04 and bHLH05 in the control of GSL levels in the absence of jasmonic acid. The combination of two specific gain-of-function alleles of MYB and bHLH proteins had an additive effect on GSL levels, as demonstrated by the analysis of the double MYB34-1D bHLH05D94N mutant, which produces 20-fold more indolic GSLs than bHLH05D94N and ecotype Columbia-0 of Arabidopsis. The amino acid substitution D94N in bHLH05D94N negatively affects the interaction with JASMONATE-ZIM DOMAIN protein, thereby resulting in constitutive activation of bHLH05 and mimicking jasmonic acid treatment. Our study revealed the bHLH04, bHLH05, and bHLH06/MYC2 factors as novel regulators of GSL biosynthesis in Arabidopsis.
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