ArticleJournal of bacteriology2014
Subcellular localization and clues for the function of the HetN factor influencing heterocyst distribution in Anabaena sp. strain PCC 7120.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Hacking heterocysts: advances in the genetic regulation of heterocyst differentiation.Archives of microbiology · 2025Review
- ThyD Is a Thylakoid Membrane Protein Influencing Cell Division and Acclimation to High Light in the Multicellular Cyanobacterium Anabaena sp. Strain PCC 7120.Molecular microbiology · 2025Article
- mRNA localization and thylakoid protein biogenesis in the filamentous heterocyst-forming cyanobacteriumJournal of bacteriology · 2024Article
- Terminal heterocyst differentiation in the Anabaena patA mutant as a result of post-transcriptional modifications and molecular leakage.PLoS computational biology · 2022Article
- Interaction network among factors involved in heterocyst-patterning in cyanobacteria.Molecular genetics and genomics : MGG · 2022Article
- Genome Analysis Coupled With Transcriptomics Reveals the Reduced Fitness of a Hot Spring CyanobacteriumFrontiers in microbiology · 2022Article
- Mathematical models of nitrogen-fixing cell patterns in filamentous cyanobacteria.Frontiers in cell and developmental biology · 2022Review
- Inactivation of Three RG(S/T)GR Pentapeptide-Containing Negative Regulators of HetR Results in Lethal Differentiation ofLife (Basel, Switzerland) · 2020Article
- HetL, HetR and PatS form a reaction-diffusion system to control pattern formation in the cyanobacteriumeLife · 2020Article
- Carbon Transfer from the Host Diatom Enables Fast Growth and High Rate of NPlants (Basel, Switzerland) · 2020Article
- Functional Dissection of Genes Encoding DNA Polymerases Based on Conditional Mutants in the Heterocyst-Forming CyanobacteriumFrontiers in microbiology · 2020Article
- Cyanobacterial Septal Junctions: Properties and Regulation.Life (Basel, Switzerland) · 2018Review
- The Ser/Thr Kinase PknH Is Essential for Maintaining Heterocyst Pattern in the CyanobacteriumLife (Basel, Switzerland) · 2018Article
- Phenotypic Assessment Suggests Multiple Start Codons for HetN, an Inhibitor of Heterocyst Differentiation, in Anabaena sp. Strain PCC 7120.Journal of bacteriology · 2018Article
- Robust stochastic Turing patterns in the development of a one-dimensional cyanobacterial organism.PLoS biology · 2018Article
- Role of PatS and cell type on the heterocyst spacing pattern in a filamentous branching cyanobacterium.FEMS microbiology letters · 2017Article
- Formation and maintenance of nitrogen-fixing cell patterns in filamentous cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Spatial fluctuations in expression of the heterocyst differentiation regulatory gene hetR in Anabaena filaments.PLoS genetics · 2015Article
- The composition of the global and feature specific cyanobacterial core-genomes.Frontiers in microbiology · 2015Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the filamentous cyanobacterium Anabaena sp. strain PCC 7120, heterocysts are formed in the absence of combined nitrogen, following a specific distribution pattern along the filament. The PatS and HetN factors contribute to the heterocyst pattern by inhibiting the formation of consecutive heterocysts. Thus, inactivation of any of these factors produces the multiple contiguous heterocyst (Mch) phenotype. Upon N stepdown, a HetN protein with its C terminus fused to a superfolder version of green fluorescent protein (sf-GFP) or to GFP-mut2 was observed, localized first throughout the whole area of differentiating cells and later specifically on the peripheries and in the polar regions of mature heterocysts, coinciding with the location of the thylakoids. Polar localization required an N-terminal stretch comprising residues 2 to 27 that may represent an unconventional signal peptide. Anabaena strains expressing a version of HetN lacking this fragment from a mutant gene placed at the native hetN locus exhibited a mild Mch phenotype. In agreement with previous results, deletion of an internal ERGSGR sequence, which is identical to the C-terminal sequence of PatS, also led to the Mch phenotype. The subcellular localization in heterocysts of fluorescence resulting from the fusion of GFP to the C terminus of HetN suggests that a full HetN protein is present in these cells. Furthermore, the full HetN protein is more conserved among cyanobacteria than the internal ERGSGR sequence. These observations suggest that HetN anchored to thylakoid membranes in heterocysts may serve a function besides that of generating a regulatory (ERGSGR) peptide.
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