Evidence map›Paper›PMID 25048800›Full record

Trial reportClinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology2015

Childhood exposure to ambient polycyclic aromatic hydrocarbons is linked to epigenetic modifications and impaired systemic immunity in T cells.

K M Hew, A I Walker, A Kohli, M Garcia, A Syed, C McDonald-Hyman, E M Noth, J K Mann, B Pratt, J Balmes and 3 more

Open access · greenAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed, 5 pooled it
9.2field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed, 5 syntheses or guidelines pooled it, 140 citations in OpenAlex.

  1. Pooled it
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  16. Article
  17. Air Pollution: Possible Interaction between the Immune and Nervous System?International journal of environmental research and public health · 2022
    Review
  18. Air pollution, metabolites and respiratory health across the life-course.European respiratory review : an official journal of the European Respiratory Society · 2022
    Review
  19. Review
  20. Article

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

K M HewDepartment of Pediatric Allergy and Immunology, Stanford University, Stanford, CA, USA.
A I Walker
A Kohli
M Garcia
A Syed
C McDonald-Hyman
E M Noth
J K Mann
B Pratt
J Balmes
S Katharine Hammond
E A Eisen
K C Nadeau
Stanford University · USBerkeley Public Health Division · USUniversity of California, Berkeley · US

Funding

SEAL (Stopping Atopic dermatitis and ALlergy) Study: Prevent allergy by enhancing the skin barrierU01AI147462 · NIAID · STANFORD UNIVERSITY · PI Mary Johnson, GIDEON LACK · 2020 to 2026
$14.0M
UC Berkeley/Stanford Children's Enviromental Health CenterP01ES022849 · NIEHS · UNIVERSITY OF CALIFORNIA BERKELEY · PI BALMES, JOHN R · 2013 to 2018
$4.2M
Phenotyping/Epigenetic studies of PAH-associated Treg impairment in asthmaR01ES020926 · NIEHS · STANFORD UNIVERSITY · PI NADEAU, KARI C. · 2012 to 2016
$2.5M
Project 3: Ambient Pollutants/Bioaerosol Effects on Treg FunctionP20ES018173 · NIEHS · UNIVERSITY OF CALIFORNIA BERKELEY · PI HAMMOND, S KATHARINE · 2010 to 2012
$895k
Modifying Regulatory T-cell signaling to enhance prevention of GVHDF30HL121873 · NHLBI · UNIVERSITY OF MINNESOTA · PI MCDONALD-HYMAN, CAMERON S · 2014 to 2017
$137k
NHLBI NIH HHS F30 HL121873NIAID NIH HHS U01 AI147462NIEHS NIH HHS EPA/NIEHS 1P01ES022849NIEHS NIH HHS EPA/NIEHS 5R01ES02092602NIEHS NIH HHS P01 ES022849NIEHS NIH HHS P20 ES018173NIEHS NIH HHS R01 ES020926
6 · The paper itself

Abstract

backgroundEvidence suggests that exposure to polycyclic aromatic hydrocarbons (PAHs) increases atopy; it is unclear how PAH exposure is linked to increased severity of atopic diseases.

objectiveWe hypothesized that ambient PAH exposure is linked to impairment of immunity in atopic children (defined as children with asthma and/or allergic rhinitis) from Fresno, California, an area with elevated ambient PAHs.

methodsWe recruited 256 subjects from Fresno, CA. Ambient PAH concentrations (ng/m(3) ) were measured using a spatial-temporal regression model over multiple time periods. Asthma diagnosis was determined by current NHLBI criteria. Phenotyping and functional immune measurements were performed from isolated cells. For epigenetic measurements, DNA was isolated and pyrosequenced.

resultsWe show that higher average PAH exposure was significantly associated with impaired Treg function and increased methylation in the forkhead box protein 3 (FOXP3) locus (P < 0.05), conditional on atopic status. These epigenetic modifications were significantly linked to differential protein expression of FOXP3 (P < 0.001). Methylation was associated with cellular functional changes, specifically Treg dysfunction, and an increase in total plasma IgE levels. Protein expression of IL-10 decreased and IFN-γ increased as the extent of PAH exposure increased. The strength of the associations generally increased as the time window for average PAH exposure increased from 24 hr to 1 year, suggesting more of a chronic response. Significant associations with chronic PAH exposure and immune outcomes were also observed in subjects with allergic rhinitis. CONCLUSIONS AND CLINICAL RELEVANCE: Collectively, these results demonstrate that increased ambient PAH exposure is associated with impaired systemic immunity and epigenetic modifications in a key locus involved in atopy: FOXP3, with a higher impact on atopic children. The results suggest that increased atopic clinical symptoms in children could be linked to increased PAH exposure in air pollution.

Indexed as

AsthmaRhinitis, AllergicAir PollutionChildChild, PreschoolDNA MethylationEnvironmental ExposureEpigenesis, GeneticFemaleForkhead Transcription FactorsHumansImmunity, CellularImmunoglobulin EInfantInterferon-gammaInterleukin-10Forkhead Transcription FactorsFOXP3 protein, humanIFNG protein, humanIL10 protein, humanImmunoglobulin EInterferon-gammaInterleukin-10Polycyclic Aromatic HydrocarbonsepigeneticsFOXP3IFN-γpolycyclic aromatic hydrocarbonstotal IgETreg functionT regulatory cells

Identifiers

PMID25048800
PMCPMC4396982
OpenAlexW2124774798

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.