ArticleBMC genomics2014
Efficient linkage mapping using exome capture and extreme QTL in schistosome parasites.
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Target gene mutation discovery and amplicon sequencing for evaluating maduramicin resistance in Eimeria tenella.Communications biology · 2025Article
- Enrichment of Helminth Mitochondrial Genomes From Faecal Samples Using Hybridisation Capture.Molecular ecology resources · 2025Article
- How useful is Nanopore adaptive sampling for sequencingWellcome open research · 2025Article
- Hybrid-Capture Target Enrichment in Human Pathogens: Identification, Evolution, Biosurveillance, and Genomic Epidemiology.Pathogens (Basel, Switzerland) · 2024Review
- Bulk segregant linkage mapping for rodent and human malaria parasites.Parasitology international · 2022Review
- Article
- Integrating genomic and epidemiologic data to accelerate progress toward schistosomiasis elimination.eLife · 2022Review
- Optimizing bulk segregant analysis of drug resistance usingiScience · 2022Article
- Genome-wide analysis of the response to ivermectin treatment by a Swedish field population of Haemonchus contortus.International journal for parasitology. Drugs and drug resistance · 2022Article
- A dense linkage map for a large repetitive genome: discovery of the sex-determining region in hybridizing fire-bellied toads (Bombina bombina and Bombina variegata).G3 (Bethesda, Md.) · 2021Article
- Genetic architecture of transmission stage production and virulence in schistosome parasites.Virulence · 2021Article
- The power and promise of genetic mapping from Plasmodium falciparum crosses utilizing human liver-chimeric mice.Communications biology · 2021Article
- Humanized Mice and the Rebirth of Malaria Genetic Crosses.Trends in parasitology · 2020Review
- Ancient Hybridization and Adaptive Introgression of an Invadolysin Gene in Schistosome Parasites.Molecular biology and evolution · 2019Article
- Genetic mapping of fitness determinants across the malaria parasite Plasmodium falciparum life cycle.PLoS genetics · 2019Article
- Extreme-QTL mapping of monepantel resistance in Haemonchus contortus.Parasites & vectors · 2019Article
- Assessing the Architecture ofG3 (Bethesda, Md.) · 2019Article
- Population genomic and evolutionary modelling analyses reveal a single major QTL for ivermectin drug resistance in the pathogenic nematode, Haemonchus contortus.BMC genomics · 2019Article
- Genetic Crosses and Linkage Mapping in Schistosome Parasites.Trends in parasitology · 2018Review
- Whole genome amplification and exome sequencing of archived schistosome miracidia.Parasitology · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIdentification of parasite genes that underlie traits such as drug resistance and host specificity is challenging using classical linkage mapping approaches. Extreme QTL (X-QTL) methods, originally developed by rodent malaria and yeast researchers, promise to increase the power and simplify logistics of linkage mapping in experimental crosses of schistosomes (or other helminth parasites), because many 1000s of progeny can be analysed, phenotyping is not required, and progeny pools rather than individuals are genotyped. We explored the utility of this method for mapping a drug resistance gene in the human parasitic fluke Schistosoma mansoni.
resultsWe staged a genetic cross between oxamniquine sensitive and resistant parasites, then between two F1 progeny, to generate multiple F2 progeny. One group of F2s infecting hamsters was treated with oxamniquine, while a second group was left untreated. We used exome capture to reduce the size of the genome (from 363 Mb to 15 Mb) and exomes from pooled F2 progeny (treated males, untreated males, treated females, untreated females) and the two parent parasites were sequenced to high read depth (mean = 95-366×) and allele frequencies at 14,489 variants compared. We observed dramatic enrichment of alleles from the resistant parent in a small region of chromosome 6 in drug-treated male and female pools (combined analysis: Z = 11.07, p = 8.74 × 10(-29)). This region contains Smp_089320 a gene encoding a sulfotransferase recently implicated in oxamniquine resistance using classical linkage mapping methods.
conclusionsThese results (a) demonstrate the utility of exome capture for generating reduced representation libraries in Schistosoma mansoni, and (b) provide proof-of-principle that X-QTL methods can be successfully applied to an important human helminth. The combination of these methods will simplify linkage analysis of biomedically or biologically important traits in this parasite.
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