ArticleInfection and immunity2014
The HtrA-like protease CD3284 modulates virulence of Clostridium difficile.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- The gram-positive HtrA, the protease that is also a chaperone.Journal of bacteriology · 2026Review
- Analysis of essential genes inJournal of bacteriology · 2025Article
- Characterization of the Clostridioides difficile 630Δerm putative Pro-Pro endopeptidase CD1597.Access microbiology · 2024Article
- Article
- E-Cadherin Orthologues as Substrates for the Serine Protease High Temperature Requirement A (HtrA).Biomolecules · 2022Article
- Novel Drivers of Virulence in Clostridioides difficile Identified via Context-Specific Metabolic Network Analysis.mSystems · 2021Article
- Complete Genome Sequencing and Comparative Phenotypic Analysis Reveal the Discrepancy BetweenFrontiers in microbiology · 2021Article
- CRISPR Genome Editing Systems in the GenusJournal of bacteriology · 2019Review
- Protease Activity ofFrontiers in cellular and infection microbiology · 2019Article
- Function of Serine Protease HtrA in the Lifecycle of the Foodborne PathogenEuropean journal of microbiology & immunology · 2018Review
- Type IV Pili Promote Clostridium difficile Adherence and Persistence in a Mouse Model of Infection.Infection and immunity · 2018Article
- Mechanistic Insights in the Success of Fecal Microbiota Transplants for the Treatment ofFrontiers in microbiology · 2018Review
- Using CRISPR-Cas9-mediated genome editing to generate C. difficile mutants defective in selenoproteins synthesis.Scientific reports · 2017Article
- HtrA Is Important for Stress Resistance and Virulence in Haemophilus parasuis.Infection and immunity · 2016Article
- Complete genome sequence of the Clostridium difficile laboratory strain 630Δerm reveals differences from strain 630, including translocation of the mobile element CTn5.BMC genomics · 2015Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
In the past decade, Clostridium difficile has emerged as an important gut pathogen. Symptoms of C. difficile infection range from mild diarrhea to pseudomembranous colitis. Besides the two main virulence factors toxin A and toxin B, other virulence factors are likely to play a role in the pathogenesis of the disease. In other Gram-positive and Gram-negative pathogenic bacteria, conserved high-temperature requirement A (HtrA)-like proteases have been shown to have a role in protein homeostasis and quality control. This affects the functionality of virulence factors and the resistance of bacteria to (host-induced) environmental stresses. We found that the C. difficile 630 genome encodes a single HtrA-like protease (CD3284; HtrA) and have analyzed its role in vivo and in vitro through the creation of an isogenic ClosTron-based htrA mutant of C. difficile strain 630Δerm (wild type). In contrast to the attenuated phenotype seen with htrA deletion in other pathogens, this mutant showed enhanced virulence in the Golden Syrian hamster model of acute C. difficile infection. Microarray data analysis showed a pleiotropic effect of htrA on the transcriptome of C. difficile, including upregulation of the toxin A gene. In addition, the htrA mutant showed reduced spore formation and adherence to colonic cells. Together, our data show that htrA can modulate virulence in C. difficile.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.