ArticleCell calcium2014
An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.
Article in Cell calcium, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
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- STIM-IP3R crosstalk regulates migration of breast cancer cells.The Journal of cell biology · 2025Article
- Systemic nimodipine affects pericyte calcium signaling, resting hemodynamics and neurovascular coupling in healthy mouse brain.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2025Article
- Two-pore channel-2 and inositol trisphosphate receptors coordinate CaCell reports · 2024Article
- Novel algorithms for improved detection and analysis of fluorescent signal fluctuations.Pflugers Archiv : European journal of physiology · 2023Article
- Dissociation of inositol 1,4,5-trisphosphate from IPThe Journal of biological chemistry · 2023Article
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- Missense mutations in inositol 1,4,5-trisphosphate receptor type 3 result in leaky CaiScience · 2022Article
- KRAP is required for diffuse and punctate IPCell calcium · 2022Article
- Functional determination of calcium-binding sites required for the activation of inositol 1,4,5-trisphosphate receptors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Segregated cation flux by TPC2 biases CaNature communications · 2022Article
- TREM2 regulates purinergic receptor-mediated calcium signaling and motility in human iPSC-derived microglia.eLife · 2022Article
- Termination of CaCell calcium · 2021Article
- Distinct movement patterns generate stages of spider web building.Current biology : CB · 2021Article
- CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors.Journal of cell science · 2021Article
- A protocol for detecting elemental calcium signals (CaSTAR protocols · 2021Article
- KRAP tethers IPNature communications · 2021Article
- Article
- Glucose and NAADP trigger elementary intracellular β-cell CaScientific reports · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Local Ca(2+) transients such as puffs and sparks form the building blocks of cellular Ca(2+) signaling in numerous cell types. They have traditionally been studied by linescan confocal microscopy, but advances in TIRF microscopy together with improved electron-multiplied CCD (EMCCD) cameras now enable rapid (>500 frames s(-1)) imaging of subcellular Ca(2+) signals with high spatial resolution in two dimensions. This approach yields vastly more information (ca. 1 Gb min(-1)) than linescan imaging, rendering visual identification and analysis of local events imaged both laborious and subject to user bias. Here we describe a routine to rapidly automate identification and analysis of local Ca(2+) events. This features an intuitive graphical user-interfaces and runs under Matlab and the open-source Python software. The underlying algorithm features spatial and temporal noise filtering to reliably detect even small events in the presence of noisy and fluctuating baselines; localizes sites of Ca(2+) release with sub-pixel resolution; facilitates user review and editing of data; and outputs time-sequences of fluorescence ratio signals for identified event sites along with Excel-compatible tables listing amplitudes and kinetics of events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.