ArticleToxicology mechanisms and methods2014
In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures.
Article in Toxicology mechanisms and methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 58 citations in OpenAlex.
- PI3K/mTOR inhibition attenuates cigarette smoke-induced senescence and SASP in oral fibroblasts: implications for tumor microenvironment remodeling.Frontiers in cell and developmental biology · 2026Article
- Exploring the Utility of Long Non-Coding RNAs for Assessing the Health Consequences of Vaping.International journal of molecular sciences · 2024Review
- Bridging systems biology and tissue engineering: Unleashing the full potential of complex 3DBiophysics reviews · 2024Review
- Oral Absorption across Organotypic Culture Models of the Human Buccal Epithelium after E-cigarette Aerosol Exposure.ACS omega · 2022Article
- Article
- Heat-not-burn tobacco (IQOS), oral fibroblasts and keratinocytes: cytotoxicity, morphological analysis, apoptosis and cellular cycle. An in vitro study.Journal of periodontal research · 2021Article
- Local Toxicity of Biocides after Direct and Aerosol Exposure on the Human Skin Epidermis and Airway Tissue Models.Toxics · 2021Article
- In vitro three-dimensional organotypic culture models of the oral mucosa.In vitro cellular & developmental biology. Animal · 2021Review
- IL-1β strengthens the physical barrier in gingival epithelial cells.Tissue barriers · 2020Article
- Investigation of multiple whole smoke dosimetry techniques using a VITROCELL®VC10® smoke exposure system.Toxicology reports · 2019Article
- Air-Liquid InterfaceApplied in vitro toxicology · 2018Review
- Development, qualification, validation and application of the Ames test using a VITROCELLToxicology reports · 2018Article
- A novel prognostic index for oral squamous cell carcinoma patients with surgically treated.Oncotarget · 2017Article
- Regulating temperature and relative humidity in air-liquid interfaceToxicology research · 2017Article
- A framework for in vitro systems toxicology assessment of e-liquids.Toxicology mechanisms and methods · 2016Article
- Enhancement of COPD biological networks using a web-based collaboration interface.F1000Research · 2015Article
- Characterization of the Vitrocell® 24/48 in vitro aerosol exposure system using mainstream cigarette smoke.Chemistry Central journal · 2014Article
- Protective Effects ofIn vivo (Athens, Greece)Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Smoking has been associated with diseases of the lung, pulmonary airways and oral cavity. Cytologic, genomic and transcriptomic changes in oral mucosa correlate with oral pre-neoplasia, cancer and inflammation (e.g. periodontitis). Alteration of smoking-related gene expression changes in oral epithelial cells is similar to that in bronchial and nasal epithelial cells. Using a systems toxicology approach, we have previously assessed the impact of cigarette smoke (CS) seen as perturbations of biological processes in human nasal and bronchial organotypic epithelial culture models. Here, we report our further assessment using in vitro human oral organotypic epithelium models. We exposed the buccal and gingival organotypic epithelial tissue cultures to CS at the air-liquid interface. CS exposure was associated with increased secretion of inflammatory mediators, induction of cytochrome P450s activity and overall weak toxicity in both tissues. Using microarray technology, gene-set analysis and a novel computational modeling approach leveraging causal biological network models, we identified CS impact on xenobiotic metabolism-related pathways accompanied by a more subtle alteration in inflammatory processes. Gene-set analysis further indicated that the CS-induced pathways in the in vitro buccal tissue models resembled those in the in vivo buccal biopsies of smokers from a published dataset. These findings support the translatability of systems responses from in vitro to in vivo and demonstrate the applicability of oral organotypical tissue models for an impact assessment of CS on various tissues exposed during smoking, as well as for impact assessment of reduced-risk products.
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