ArticleJournal of visualized experiments : JoVE2014
Production of haploid zebrafish embryos by in vitro fertilization.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Polystyrene nanoplastics: optimized removal using magnetic nano-adsorbent and toxicity assessment in zebrafish embryos.Journal of environmental health science & engineering · 2024Article
- Quality of fish eggs and production of androgenetic and gynogenetic doubled haploids (DHs).Fish physiology and biochemistry · 2024Review
- Haploidy-linked cell proliferation defects limit larval growth in zebrafish.Open biology · 2024Article
- Post-meiotic mechanism of facultative parthenogenesis in gonochoristic whiptail lizard species.eLife · 2024Article
- (Zebra)fishing for nephrogenesis genes.Tissue barriers · 2024Review
- Zebrafish as a Model to Study Retinoic Acid Signaling in Development and Disease.Biomedicines · 2023Review
- Principles of Zebrafish Nephron Segment Development.Journal of developmental biology · 2023Review
- Modeling Podocyte Ontogeny and Podocytopathies with the Zebrafish.Journal of developmental biology · 2023Review
- Article
- Shutdown corner, a large deletion mutant isolated from a haploid mutagenesis screen in zebrafish.G3 (Bethesda, Md.) · 2022Article
- Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants.Journal of visualized experiments : JoVE · 2021Article
- Chromatin architecture transitions from zebrafish sperm through early embryogenesis.Genome research · 2021Article
- Zinc protection of fertilized eggs is an ancient feature of sexual reproduction in animals.PLoS biology · 2020Article
- Chromatin dynamics at the maternal to zygotic transition: recent advances from the zebrafish model.F1000Research · 2020Review
- Tfap2a is a novel gatekeeper of nephron differentiation during kidney development.Development (Cambridge, England) · 2019Article
- Brd4 and P300 Confer Transcriptional Competency during Zygotic Genome Activation.Developmental cell · 2019Article
- Zebrafish embryonic development is induced by carp sperm.Biology letters · 2016Article
- Microbead Implantation in the Zebrafish Embryo.Journal of visualized experiments : JoVE · 2015Article
- Review
- Nephron proximal tubule patterning and corpuscles of Stannius formation are regulated by the sim1a transcription factor and retinoic acid in zebrafish.Developmental biology · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The zebrafish has become a mainstream vertebrate model that is relevant for many disciplines of scientific study. Zebrafish are especially well suited for forward genetic analysis of developmental processes due to their external fertilization, embryonic size, rapid ontogeny, and optical clarity--a constellation of traits that enable the direct observation of events ranging from gastrulation to organogenesis with a basic stereomicroscope. Further, zebrafish embryos can survive for several days in the haploid state. The production of haploid embryos in vitro is a powerful tool for mutational analysis, as it enables the identification of recessive mutant alleles present in first generation (F1) female carriers following mutagenesis in the parental (P) generation. This approach eliminates the necessity to raise multiple generations (F2, F3, etc.) which involves breeding of mutant families, thus saving the researcher time along with reducing the needs for zebrafish colony space, labor, and the husbandry costs. Although zebrafish have been used to conduct forward screens for the past several decades, there has been a steady expansion of transgenic and genome editing tools. These tools now offer a plethora of ways to create nuanced assays for next generation screens that can be used to further dissect the gene regulatory networks that drive vertebrate ontogeny. Here, we describe how to prepare haploid zebrafish embryos. This protocol can be implemented for novel future haploid screens, such as in enhancer and suppressor screens, to address the mechanisms of development for a broad number of processes and tissues that form during early embryonic stages.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.