ArticleJournal of visualized experiments : JoVE2014
Collection, isolation, and flow cytometric analysis of human endocervical samples.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 24 citations in OpenAlex.
- Using safe, affordable and accessible non-steroidal anti-inflammatory drugs to reduce the number of HIV target cells in the blood and at the female genital tract.Journal of the International AIDS Society · 2018Trial
- Improving Adherence to Post-Cervical Biopsy Sexual Abstinence in Kenyan Female Sex Workers.American journal of reproductive immunology (New York, N.Y. : 1989) · 2016Trial
- HIV-Exposed Seronegative Female Sex Workers Show Different Cellular Immune Profiles Across the Menstrual Cycle.American journal of reproductive immunology (New York, N.Y. : 1989) · 2025Article
- Salivary Lymphocyte Phenotypes Differ from Blood and Serve as a Model for Other Mucosal Fluids.Journal of cellular immunology · 2025Article
- Viral and immune dynamics of genital human papillomavirus infections in young women with high temporal resolution.PLoS biology · 2025Observational
- A Noninvasive Method to Sample Immune Cells in the Lower Female Genital Tract Using Menstrual Discs.ImmunoHorizons · 2024Article
- Article
- Preserved Mucosal-Associated Invariant T Cells in the Cervical Mucosa of HIV-Infected Women with Dominant Expression of the TRAV1-2-TRAJ20 T Cell Receptor α-Chain.The Journal of infectious diseases · 2022Article
- HIV-Exposed Seronegative Sex Workers Express Low T-Cell Activation and an Intact Ectocervical Tissue Microenvironment.Vaccines · 2021Article
- Low-Dose Acetylsalicylic Acid Reduces T Cell Immune Activation: Potential Implications for HIV Prevention.Frontiers in immunology · 2021Article
- Differential Elevation of Inflammation and CD4Frontiers in immunology · 2020Article
- Article
- Increased Cervical CD4AIDS research and human retroviruses · 2019Article
- Characterization of the Genital Mucosa Immune Profile to Distinguish Phases of the Menstrual Cycle: Implications for HIV Susceptibility.The Journal of infectious diseases · 2019Article
- Isolation of Dendritic Cells from the Human Female Reproductive Tract for Phenotypical and Functional Studies.Journal of visualized experiments : JoVE · 2018Article
- CD161 identifies polyfunctional Th1/Th17 cells in the genital mucosa that are depleted in HIV-infected female sex workers from Nairobi, Kenya.Scientific reports · 2017Article
- Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells.Journal of visualized experiments : JoVE · 2017Article
- Minimally invasive monitoring of CD4 T cells at multiple mucosal tissues after intranasal vaccination in rhesus macaques.PloS one · 2017Article
- Association of high-risk sexual behaviour with diversity of the vaginal microbiota and abundance of Lactobacillus.PloS one · 2017Article
- Loss of Intra-Epithelial Endocervical Gamma Delta (GD) 1 T Cells in HIV-Infected Women.American journal of reproductive immunology (New York, N.Y. : 1989) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Despite the public health importance of mucosal pathogens (including HIV), relatively little is known about mucosal immunity, particularly at the female genital tract (FGT). Because heterosexual transmission now represents the dominant mechanism of HIV transmission, and given the continual spread of sexually transmitted infections (STIs), it is critical to understand the interplay between host and pathogen at the genital mucosa. The substantial gaps in knowledge around FGT immunity are partially due to the difficulty in successfully collecting and processing mucosal samples. In order to facilitate studies with sufficient sample size, collection techniques must be minimally invasive and efficient. To this end, a protocol for the collection of cervical cytobrush samples and subsequent isolation of cervical mononuclear cells (CMC) has been optimized. Using ex vivo flow cytometry-based immunophenotyping, it is possible to accurately and reliably quantify CMC lymphocyte/monocyte population frequencies and phenotypes. This technique can be coupled with the collection of cervical-vaginal lavage (CVL), which contains soluble immune mediators including cytokines, chemokines and anti-proteases, all of which can be used to determine the anti- or pro-inflammatory environment in the vagina.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.