ArticleJournal of visualized experiments : JoVE2014
Multi-step preparation technique to recover multiple metabolite compound classes for in-depth and informative metabolomic analysis.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed.
- Insufficient sleep and weekend recovery sleep: classification by a metabolomics-based machine learning ensemble.Scientific reports · 2023Trial
- Trial
- Identification of a Preliminary Plasma Metabolome-based Biomarker for Circadian Phase in Humans.Journal of biological rhythms · 2021Trial
- Internal Circadian Misalignment of the Human Metabolome Links Night Shiftwork to Metabolic Impairment.Journal of biological rhythms · 2026Article
- Induced Sputum Multi-Omics Reveals Airway Signatures of COPD in Smokers: A Pilot Study.International journal of molecular sciences · 2026Article
- Characterization of astaxanthin isomers in different types of salmon filets and human plasma after salmon consumption.Food chemistry · 2025Article
- Opioid quantification via microsampling techniques to assess opioid use in human laboratory studies.Scientific reports · 2025Article
- Development of an HPLC-UV method for quantification of posaconazole in low-volume plasma samples: design of experiments and machine learning models.BMC chemistry · 2024Article
- Metabolomics Profiling of White Button, Crimini, Portabella, Lion's Mane, Maitake, Oyster, and Shiitake Mushrooms Using Untargeted Metabolomics and Targeted Amino Acid Analysis.Foods (Basel, Switzerland) · 2023Article
- Multi-Omics Analysis of Lung Tissue Demonstrates Changes to Lipid Metabolism during Allergic Sensitization in Mice.Metabolites · 2023Article
- Transcriptome and metabolome changes induced by bitter melon (Journal of traditional and complementary medicine · 2022Article
- UPLC-ESI-MS/MS Based Characterization of Active Flavonoids fromAntioxidants (Basel, Switzerland) · 2021Article
- Comparing the effects of an exposure to a polycyclic aromatic hydrocarbon mixture versus individual polycyclic aromatic hydrocarbons during monocyte to macrophage differentiation: Mixture exposure results in altered immune metrics.Journal of applied toxicology : JAT · 2021Article
- Lipidomics-Based Comparison of Molecular Compositions of Green, Yellow, and Red Bell Peppers.Metabolites · 2021Article
- Towards Higher Sensitivity of Mass Spectrometry: A Perspective From the Mass Analyzers.Frontiers in chemistry · 2021Review
- Developing preliminary blood metabolomics-based biomarkers of insufficient sleep in humans.Sleep · 2020Article
- Untargeted metabolite profiling on the water-soluble metabolites of edible bird's nest through liquid chromatography-mass spectrometry.Veterinary world · 2020Article
- Putative multiple reaction monitoring strategy for the comparative pharmacokinetics of postoral administration Renshen-Yuanzhi compatibility through liquid chromatography-tandem mass spectrometry.Journal of ginseng research · 2020Article
- Bronchoalveolar Lavage Fluid from COPD Patients Reveals More Compounds Associated with Disease than Matched Plasma.Metabolites · 2019Article
- Early Mechanistic Events Induced by Low Molecular Weight Polycyclic Aromatic Hydrocarbons in Mouse Lung Epithelial Cells: A Role for Eicosanoid Signaling.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Metabolomics is an emerging field which enables profiling of samples from living organisms in order to obtain insight into biological processes. A vital aspect of metabolomics is sample preparation whereby inconsistent techniques generate unreliable results. This technique encompasses protein precipitation, liquid-liquid extraction, and solid-phase extraction as a means of fractionating metabolites into four distinct classes. Improved enrichment of low abundance molecules with a resulting increase in sensitivity is obtained, and ultimately results in more confident identification of molecules. This technique has been applied to plasma, bronchoalveolar lavage fluid, and cerebrospinal fluid samples with volumes as low as 50 µl. Samples can be used for multiple downstream applications; for example, the pellet resulting from protein precipitation can be stored for later analysis. The supernatant from that step undergoes liquid-liquid extraction using water and strong organic solvent to separate the hydrophilic and hydrophobic compounds. Once fractionated, the hydrophilic layer can be processed for later analysis or discarded if not needed. The hydrophobic fraction is further treated with a series of solvents during three solid-phase extraction steps to separate it into fatty acids, neutral lipids, and phospholipids. This allows the technician the flexibility to choose which class of compounds is preferred for analysis. It also aids in more reliable metabolite identification since some knowledge of chemical class exists.
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Registered trials
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