ArticleThe EMBO journal1989
A 14 bp promoter element directs the testis specificity of the Drosophila beta 2 tubulin gene.
Article in The EMBO journal, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 128 citations in OpenAlex.
- Article
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- Derepression of Y-linked multicopy protamine-like genes interferes with sperm nuclear compaction inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology.International journal of molecular sciences · 2022Article
- Regulation of Tubulin Gene Expression: From Isotype Identity to Functional Specialization.Frontiers in cell and developmental biology · 2022Review
- The βScientific reports · 2021Article
- Article
- Transcriptional Enhancers inGenetics · 2020Review
- Developmental regulation of cell type-specific transcription by novel promoter-proximal sequence elements.Genes & development · 2020Article
- Improvement and use of CRISPR/Cas9 to engineer a sperm-marking strain for the invasive fruit pest Drosophila suzukii.BMC biotechnology · 2019Article
- Differentially and Co-expressed Genes in Embryo, Germ-Line and Somatic Tissues ofG3 (Bethesda, Md.) · 2019Article
- MNM and SNM maintain but do not establish achiasmate homolog conjunction during Drosophila male meiosis.PLoS genetics · 2019Article
- Evidence for a hierarchical transcriptional circuit in Drosophila male germline involving testis-specific TAF and two gene-specific transcription factors, Mod and Acj6.FEBS letters · 2018Article
- A Splice Variant of Centrosomin Converts Mitochondria to Microtubule-Organizing Centers.Current biology : CB · 2017Article
- Rapid Functional and Sequence Differentiation of a Tandemly Repeated Species-Specific Multigene Family in Drosophila.Molecular biology and evolution · 2017Article
- Highly efficient Cas9-mediated gene drive for population modification of the malaria vector mosquito Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Relocation facilitates the acquisition of short cis-regulatory regions that drive the expression of retrogenes during spermatogenesis in Drosophila.Molecular biology and evolution · 2014Article
- Distinct genetic programs guide Drosophila circular and longitudinal visceral myoblast fusion.BMC cell biology · 2014Article
- Male-specific Y-linked transgene markers to enhance biologically-based control of the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae).BMC genetics · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To analyze the regulation of gene expression during male germ cell development, we investigated the testis-specific expression of the Drosophila beta 2 tubulin gene. Germ line transformation experiments with the upstream region of the D.melanogaster beta 2 tubulin gene fused to the Escherichia coli lacZ gene resulted in the correct tissue specific expression of the reporter gene. Furthermore, we showed that the upstream sequences of the beta 2 tubulin gene of the distantly related species D.hydei can drive the expression of the lacZ gene testis specifically in D.melanogaster flies. A detailed deletion analysis showed that 53 bp of upstream and 23 bp (D.melanogaster) or 29 bp (D.hydei) of leader sequences are sufficient to confer tissue specificity. The short promoter regions contain a 14 bp motif at identical positions in both species, which acts as a position-dependent promoter element. In vitro mutagenesis and subsequent germline transformation experiments revealed that this sequence is the only element necessary for the testis-specific transcription of the beta 2 tubulin gene in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.