ArticleJournal of mass spectrometry : JMS2014
A derivatization and validation strategy for determining the spatial localization of endogenous amine metabolites in tissues using MALDI imaging mass spectrometry.
Article in Journal of mass spectrometry : JMS, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Chemical Derivatization Strategies for Expanding Small Biomolecule Analysis by MALDI-MS.Metabolites · 2026Review
- MALDI mass spectrometry imaging of extracellular matrix proteins.The Analyst · 2025Review
- Application of a Multiomics Imaging Workflow to Explore Asparlas Treatment in Solid Tumors.Analytical chemistry · 2025Article
- Imaging plant metabolism in situ.Journal of experimental botany · 2024Review
- On-tissue chemical derivatization in mass spectrometry imaging.Mass spectrometry reviews · 2022Review
- Nanosecond Photochemical Reaction for Enhanced Identification, Quantification, and Visualization of Primary Amine-Containing Metabolites by MALDI-Mass Spectrometry.Analytical chemistry · 2022Article
- Applications of spatially resolved omics in the field of endocrine tumors.Frontiers in endocrinology · 2022Review
- Enhancing Metabolite Coverage for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging Through Multiple On-Tissue Chemical Derivatizations.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nanosecond Photochemical Reaction (nsPCR) for Enhanced Mass Spectrometric Identification, Quantification, and Visualization of Metabolites and Neuropeptides.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Molecular cartography of residue pesticides on grape surface in 3D by ambient ionization tandem mass spectrometry.Journal of food and drug analysis · 2021Article
- Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging.Nature protocols · 2021Article
- The Study of Derivatization Prior MALDI MSI Analysis-Charge Tagging Based on the Cholesterol and Betaine Aldehyde.Molecules (Basel, Switzerland) · 2021Article
- MALDI Matrices for the Analysis of Low Molecular Weight Compounds: Rational Design, Challenges and Perspectives.Chemistry, an Asian journal · 2021Review
- Recent developments of novel matrices and on-tissue chemical derivatization reagents for MALDI-MSI.Analytical and bioanalytical chemistry · 2021Review
- An optimized method for the detection and spatial distribution of aminoglycoside and vancomycin antibiotics in tissue sections by mass spectrometry imaging.Journal of mass spectrometry : JMS · 2021Article
- Advances in MALDI Mass Spectrometry Imaging Single Cell and Tissues.Frontiers in chemistry · 2021Review
- Single-Cell Metabolomics by Mass Spectrometry Imaging.Advances in experimental medicine and biology · 2021Article
- Integrating ion mobility and imaging mass spectrometry for comprehensive analysis of biological tissues: A brief review and perspective.Journal of mass spectrometry : JMS · 2020Article
- Visualizing Genotypic and Developmental Differences of Free Amino Acids in Maize Roots With Mass Spectrometry Imaging.Frontiers in plant science · 2020Article
- Use of mTRAQ derivatization reagents on tissues for imaging neurotransmitters by MALDI imaging mass spectrometry: the triple spray method.Analytical and bioanalytical chemistry · 2019Article
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5 authors.
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Abstract
Imaging mass spectrometry (IMS) studies increasingly focus on endogenous small molecular weight metabolites and consequently bring special analytical challenges. Since analytical tissue blanks do not exist for endogenous metabolites, careful consideration must be given to confirm molecular identity. Here, we present approaches for the improvement in detection of endogenous amine metabolites such as amino acids and neurotransmitters in tissues through chemical derivatization and matrix-assisted laser desorption/ionization (MALDI) IMS. Chemical derivatization with 4-hydroxy-3-methoxycinnamaldehyde (CA) was used to improve sensitivity and specificity. CA was applied to the tissue via MALDI sample targets precoated with a mixture of derivatization reagent and ferulic acid as a MALDI matrix. Spatial distributions of chemically derivatized endogenous metabolites in tissue were determined by high-mass resolution and MS(n) IMS. We highlight an analytical strategy for metabolite validation whereby tissue extracts are analyzed by high-performance liquid chromatography (HPLC)-MS/MS to unambiguously identify metabolites and distinguish them from isobaric compounds.
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