ArticleCytoskeleton (Hoboken, N.J.)2014
Oscillatory fluid flow influences primary cilia and microtubule mechanics.
Article in Cytoskeleton (Hoboken, N.J.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Mechanobiological Response of Osteocyte TRPV4 Base on the Piezoelectricity of Bone Matrix.Annals of biomedical engineering · 2026Article
- Mechanobiology and Primary Cilium in the Pathophysiology of Bone Marrow Myeloproliferative Diseases.International journal of molecular sciences · 2024Review
- High water intake induces primary cilium elongation in renal tubular cells.Kidney research and clinical practice · 2024Article
- Cold-shock proteins accumulate in centrosomes and their expression and primary cilium morphology are regulated by hypothermia and shear stress.Histology and histopathology · 2024Article
- Osteoclastogenesis Requires Primary Cilia Disassembly and Can Be Inhibited by Promoting Primary Cilia Formation Pharmacologically.Cells, tissues, organs · 2024Article
- Osteocytes and Primary Cilia.Current osteoporosis reports · 2023Review
- Review
- Review
- The Diminishing Returns of Mechanical Loading and Potential Mechanisms that Desensitize Osteocytes.Current osteoporosis reports · 2021Review
- The effects of locomotion on bone marrow mesenchymal stem cell fate: insight into mechanical regulation and bone formation.Cell & bioscience · 2021Review
- Estrogen withdrawal alters cytoskeletal and primary ciliary dynamics resulting in increased Hedgehog and osteoclastogenic paracrine signalling in osteocytes.Scientific reports · 2021Article
- Primary cilium: a paradigm for integrating mathematical modeling with experiments and numerical simulations in mechanobiology.Mathematical biosciences and engineering : MBE · 2021Article
- Primary cilia mediate Klf2-dependant Notch activation in regenerating heart.Protein & cell · 2020Article
- Effects of FSS on the expression and localization of the core proteins in two Wnt signaling pathways, and their association with ciliogenesis.International journal of molecular medicine · 2018Article
- TRPV4-mediates oscillatory fluid shear mechanotransduction in mesenchymal stem cells in part via the primary cilium.Scientific reports · 2018Article
- Microtubules tune mechanotransduction through NOX2 and TRPV4 to decrease sclerostin abundance in osteocytes.Science signaling · 2017Article
- Primary cilia: Cell and molecular mechanosensors directing whole tissue function.Seminars in cell & developmental biology · 2017Review
- Microgravity induces inhibition of osteoblastic differentiation and mineralization through abrogating primary cilia.Scientific reports · 2017Article
- Novel multi-functional fluid flow device for studying cellular mechanotransduction.Journal of biomechanics · 2016Article
- The molecular basis of bone mechanotransduction.Journal of musculoskeletal & neuronal interactions · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Many tissues are sensitive to mechanical stimuli; however, the mechanotransduction mechanism used by cells remains unknown in many cases. The primary cilium is a solitary, immotile microtubule-based extension present on nearly every mammalian cell which extends from the basal body. The cilium is a mechanosensitive organelle and has been shown to transduce fluid flow-induced shear stress in tissues, such as the kidney and bone. The majority of microtubules assemble from the mother centriole (basal body), contributing significantly to the anchoring of the primary cilium. Several studies have attempted to quantify the number of microtubules emanating from the basal body and the results vary depending on the cell type. It has also been shown that cellular response to shear stress depends on microtubular integrity. This study hypothesizes that changing the microtubule attachment of primary cilia in response to a mechanical stimulus could change primary cilia mechanics and, possibly, mechanosensitivity. Oscillatory fluid flow was applied to two different cell types and the microtubule attachment to the ciliary base was quantified. For the first time, an increase in microtubules around primary cilia both with time and shear rate in response to oscillatory fluid flow stimulation was demonstrated. Moreover, it is presented that the primary cilium is required for this loading-induced cellular response. This study has demonstrated a new role for the cilium in regulating alterations in the cytoplasmic microtubule network in response to mechanical stimulation, and therefore provides a new insight into how cilia may regulate its mechanics and thus the cells mechanosensitivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.