ArticleAngewandte Chemie (International ed. in English)2014
Using the population-shift mechanism to rationally introduce "Hill-type" cooperativity into a normally non-cooperative receptor.
Article in Angewandte Chemie (International ed. in English), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- On the Rational Design of Cooperative Receptors.Annual review of biophysics · 2023Review
- The sequestration mechanism as a generalizable approach to improve the sensitivity of biosensors and bioassays.Chemical science · 2022Article
- Integrating PCR-free amplification and synergistic sensing for ultrasensitive and rapid CRISPR/Cas12a-based SARS-CoV-2 antigen detection.Synthetic and systems biotechnology · 2021Article
- Multiple sensors provide spatiotemporal oxygen regulation of gene expression in a Rhizobium-legume symbiosis.PLoS genetics · 2021Article
- Thermodynamic analysis of cooperative ligand binding by the ATP-binding DNA aptamer indicates a population-shift binding mechanism.Scientific reports · 2020Article
- Rational design to control the trade-off between receptor affinity and cooperativity.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Re-Evaluating the Conventional Wisdom about Binding Assays.Trends in biochemical sciences · 2020Review
- Innovative engineering and sensing strategies for aptamer-based small-molecule detection.Trends in analytical chemistry : TRAC · 2019Article
- Electrochemical DNA-Based Sensors for Molecular Quality Control: Continuous, Real-Time Melamine Detection in Flowing Whole Milk.Analytical chemistry · 2018Article
- First characterization of a biphasic, switch-like DNA amplification.The Analyst · 2018Article
- New insights into a classic aptamer: binding sites, cooperativity and more sensitive adenosine detection.Nucleic acids research · 2017Article
- A cooperative-binding split aptamer assay for rapid, specific and ultra-sensitive fluorescence detection of cocaine in saliva.Chemical science · 2017Article
- A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS).The Analyst · 2016Article
- Using Nature's "Tricks" To Rationally Tune the Binding Properties of Biomolecular Receptors.Accounts of chemical research · 2016Review
- Principles of allosteric interactions in cell signaling.Journal of the American Chemical Society · 2014Review
- Intrinsic disorder as a generalizable strategy for the rational design of highly responsive, allosterically cooperative receptors.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
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5 authors.
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Abstract
Allosteric cooperativity, which nature uses to improve the sensitivity with which biomolecular receptors respond to small changes in ligand concentration, could likewise be of use in improving the responsiveness of artificial biosystems. Thus motivated, we demonstrate here the rational design of cooperative molecular beacons, a widely employed DNA sensor, using a generalizable population-shift approach in which we engineer receptors that equilibrate between a low-affinity state and a high-affinity state exposing two binding sites. Doing so we achieve cooperativity within error of ideal behavior, greatly steepening the beacon's binding curve relative to that of the parent receptor. The ability to rationally engineer cooperativity should prove useful in applications such as biosensors, synthetic biology and "smart" biomaterials, in which improved responsiveness is of value.
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