ArticleThe Prostate2014
Canine prostate cancer cell line (Probasco) produces osteoblastic metastases in vivo.
Article in The Prostate, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 40 citations in OpenAlex.
- A review of spontaneous bone metastases in domestic and laboratory animals.Journal of bone oncology · 2026Review
- Establishment and Characterisation of Two Canine Prostate Cancer Cell Lines with Stem Cell Marker Expression.Animals : an open access journal from MDPI · 2026Article
- Pathology of a Canine Model of Localized Prostate Carcinoma.The Prostate · 2026Article
- Genomic Evaluation of Canine Prostatic Carcinomas as a Model for the Human Disease: or 'UC or not UC - that is the question'.Veterinary and comparative oncology · 2025Article
- Cancer metastasis to the bone: Mechanisms and animal models (Review).Oncology letters · 2025Review
- Article
- Advances in organoid technology for veterinary disease modeling.Frontiers in veterinary science · 2023Review
- Investigating PSMA differential expression in canine uroepithelial carcinomas to aid disease-based stratification and guide therapeutic selection.BMC veterinary research · 2022Article
- RNA-seq of nine canine prostate cancer cell lines reveals diverse therapeutic target signatures.Cancer cell international · 2022Article
- Comparative pathology of dog and human prostate cancer.Veterinary medicine and science · 2022Review
- Article
- Canine prostatic cancer cell line (LuMa) with osteoblastic bone metastasis.The Prostate · 2020Article
- Parathyroid hormone-related protein promotes bone loss in T-cell leukemia as well as in solid tumors.Leukemia & lymphoma · 2020Article
- Establishment and characterization of stable red, far-red (fR) and near infra-red (NIR) transfected canine prostate cancer cell lines.Cancer cell international · 2020Article
- Article
- Mouse model recapitulates the phenotypic heterogeneity of human adult T-cell leukemia/lymphoma in bone.Journal of bone oncology · 2019Article
- Suitability of ultrasound-guided fine-needle aspiration biopsy for transcriptome sequencing of the canine prostate.Scientific reports · 2019Article
- Comprehensive Genomic Profiling of Androgen-Receptor-Negative Canine Prostate Cancer.International journal of molecular sciences · 2019Article
- Emerging and Established Models of Bone Metastasis.Cancers · 2018Review
- Intra-iliac Artery Injection for Efficient and Selective Modeling of Microscopic Bone Metastasis.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIn 2012, over 240,000 men were diagnosed with prostate cancer and over 28,000 died from the disease. Animal models of prostate cancer are vital to understanding its pathogenesis and developing therapeutics. Canine models in particular are useful due to their similarities to late-stage, castration-resistant human disease with osteoblastic bone metastases. This study established and characterized a novel canine prostate cancer cell line that will contribute to the understanding of prostate cancer pathogenesis.
methodsA novel cell line (Probasco) was derived from a mixed breed dog that had spontaneous prostate cancer. Cell proliferation and motility were analyzed in vitro. Tumor growth in vivo was studied by subcutaneous, intratibial, and intracardiac injection of Probasco cells into nude mice. Tumors were evaluated by bioluminescent imaging, Faxitron radiography, µCT, and histology. RT-PCR and genome-wide DNA copy number profiling were used to characterize the cell line.
resultsThe Probasco cells grew in vitro (over 75 passages) and were tumorigenic in nude mice. Probasco cells expressed high levels of BMP2, CDH1, MYOF, FOLH1, RUNX2, and SMAD5 modest CXCL12, SLUG, and BMP, and no PTHrP mRNA. Following intracardiac injection, Probasco cells metastasized primarily to the appendicular skeleton, and both intratibial and intracardiac injections produced osteoblastic tumors in bone. Comparative genomic hybridization demonstrated numerous DNA copy number aberrations throughout the genome, including large losses and gains in multiple chromosomes.
conclusionsThe Probasco prostate cancer cell line will be a valuable model to investigate the mechanisms of prostate cancer pathogenesis and osteoblastic bone metastases.
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Registered trials
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