ArticleNature2014
Serial time-resolved crystallography of photosystem II using a femtosecond X-ray laser.
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Article in Nature, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 144 papers.
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144citing papers in PubMed
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144 citing papers in PubMed.
- Pathway(s) and distribution of photosystem II-cyclic electron flow.Biochemical Society transactions · 2026Review
- Capturing irreversible photoswitching inChemical communications (Cambridge, England) · 2026Article
- Plant structural biology: Emerging technologies and future biological insights.The Plant journal : for cell and molecular biology · 2026Review
- A Bright and Dynamic Future in Structural Biology.Advances in experimental medicine and biology · 2026Review
- Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026Review
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Capturing structural intermediates in an animal-like cryptochrome photoreceptor by time-resolved crystallography.Science advances · 2025Article
- Effect of crystal-to-detector distance shift on data processing in serial crystallography.PloS one · 2025Article
- Structural effects of high laser power densities on an early bacteriorhodopsin photocycle intermediate.Nature communications · 2024Article
- Small-rotative fixed-target serial synchrotron crystallography (SR-FT-SSX) for molecular crystals.Communications chemistry · 2024Article
- Bicarbonate is a key regulator but not a substrate for OPhotosynthesis research · 2024Review
- Article
- Theoretical Studies of Anisotropic Melting of Ice Induced by Ultrafast Nonthermal Heating.ACS physical chemistry Au · 2024Article
- Mutation-induced shift of the photosystem II active site reveals insight into conserved water channels.The Journal of biological chemistry · 2024Article
- A comparative review of time-resolved x-ray and electron scattering to probe structural dynamics.Structural dynamics (Melville, N.Y.) · 2024Review
- Dynamic three-dimensional structures of a metal-organic framework captured with femtosecond serial crystallography.Nature chemistry · 2024Article
- The time revolution in macromolecular crystallography.Structural dynamics (Melville, N.Y.) · 2024Article
- Review of serial femtosecond crystallography including the COVID-19 pandemic impact and future outlook.Structure (London, England : 1993) · 2023Review
- Does Serial Femtosecond Crystallography Depict State-Specific Catalytic Intermediates of the Oxygen-Evolving Complex?Journal of the American Chemical Society · 2023Article
- Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics.Annual review of biophysics · 2023Review
84 more citing papers are in PubMed but not listed here.
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Authors and funding
59 authors.
Christopher Kupitz1] Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA [2].
Shibom Basu1] Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA [2].
Ingo GrotjohannDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Raimund FrommeDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Nadia A ZatsepinDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Kimberly N RendekDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Mark S Hunter1] Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA [2] Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Robert L ShoemanMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.
Thomas A WhiteCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Dingjie WangDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Daniel JamesDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Jay-How YangDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Danielle E CobbDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Brenda ReederDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Raymond G SierraStanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Haiguang LiuDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Anton BartyCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Andrew L Aquila1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] European XFEL GmbH, Notkestrasse 85, 22607 Hamburg, Germany.
Daniel Deponte1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Richard A Kirian1] Department of Physics, Arizona State University, Tempe, Arizona 85287, USA [2] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Sadia Bari1] Max Planck Advanced Study Group, Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany [2] Max-Planck-Institut für Kernphysik, Saupfercheckweg 1, 69117 Heidelberg, Germany.
Jesse J BergkampDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Kenneth R BeyerleinCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Michael J BoganStanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Carl Caleman1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics and Astronomy, Uppsala University, Regementsvägen 1, SE-752 37 Uppsala, Sweden.
Tzu-Chiao Chao1] Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA [2] University of Regina, 3737 Wascana Pkwy Regina, Saskatchewan S4S 0A2, Canada.
Chelsie E ConradDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Katherine M DavisDepartment of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, Indiana 47907, USA.
Holger FleckensteinCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Lorenzo Galli1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
Stefan P Hau-RiegeLawrence Livermore National Laboratory, Livermore, California 94550, USA.
Stephan Kassemeyer1] Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Max Planck Advanced Study Group, Center for Free-Electron Laser Science (CFEL), Notkestrasse 85, 22607 Hamburg, Germany.
Hartawan LaksmonoStanford PULSE Institute, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Mengning LiangCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Lukas LombMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.
Stefano MarchesiniLawrence Berkeley National Laboratory, Berkeley, California 94720, USA.
Andrew V Martin1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department ARC Centre of Excellence for Coherent X-ray Science, Department of Physics, University of Melbourne, Parkville VIC 3010, Australia.
Marc MesserschmidtLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Despina MilathianakiLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Karol Nass1] Max-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany [2] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [3] University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany.
Alexandra RosDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Shatabdi Roy-ChowdhuryDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Kevin SchmidtDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Marvin Seibert1] Linac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA [2] Uppsala University, Sankt Olofsgatan 10B, 753 12 Uppsala, Sweden.
Jan SteinbrenerMax-Planck-Institut für medizinische Forschung, Jahnstrasse 29, 69120 Heidelberg, Germany.
Francesco StellatoCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Lifen YanDepartment of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, Indiana 47907, USA.
Chunhong Yoon1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] European XFEL GmbH, Notkestrasse 85, 22607 Hamburg, Germany.
Thomas A MooreDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Ana L MooreDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Yulia PushkarDepartment of Physics, Purdue University, 525 Northwestern Avenue, West Lafayette, Indiana 47907, USA.
Garth J WilliamsLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Sébastien BoutetLinac Coherent Light Source, Stanford Linear Accelerator Center (SLAC) National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
R Bruce DoakDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Uwe WeierstallDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Matthias FrankLawrence Livermore National Laboratory, Livermore, California 94550, USA.
Henry N Chapman1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] University of Hamburg, Luruper Chaussee 149, 22761 Hamburg, Germany [3] Center for Ultrafast Imaging, Luruper Chaussee 149, 22761 Hamburg, Germany.
John C H SpenceDepartment of Physics, Arizona State University, Tempe, Arizona 85287, USA.
Petra FrommeDepartment of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
Funding
Femtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3MNIGMS NIH HHS 1R01GM095583NIGMS NIH HHS R01 GM095583
Abstract
Photosynthesis, a process catalysed by plants, algae and cyanobacteria converts sunlight to energy thus sustaining all higher life on Earth. Two large membrane protein complexes, photosystem I and II (PSI and PSII), act in series to catalyse the light-driven reactions in photosynthesis. PSII catalyses the light-driven water splitting process, which maintains the Earth's oxygenic atmosphere. In this process, the oxygen-evolving complex (OEC) of PSII cycles through five states, S0 to S4, in which four electrons are sequentially extracted from the OEC in four light-driven charge-separation events. Here we describe time resolved experiments on PSII nano/microcrystals from Thermosynechococcus elongatus performed with the recently developed technique of serial femtosecond crystallography. Structures have been determined from PSII in the dark S1 state and after double laser excitation (putative S3 state) at 5 and 5.5 Å resolution, respectively. The results provide evidence that PSII undergoes significant conformational changes at the electron acceptor side and at the Mn4CaO5 core of the OEC. These include an elongation of the metal cluster, accompanied by changes in the protein environment, which could allow for binding of the second substrate water molecule between the more distant protruding Mn (referred to as the 'dangler' Mn) and the Mn3CaOx cubane in the S2 to S3 transition, as predicted by spectroscopic and computational studies. This work shows the great potential for time-resolved serial femtosecond crystallography for investigation of catalytic processes in biomolecules.
Indexed as
Crystallography, X-RayModels, MolecularCyanobacteriaPhotosystem II Protein ComplexProtein Structure, TertiaryPhotosystem II Protein Complex
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