ArticleMolecular microbiology2014
NilD CRISPR RNA contributes to Xenorhabdus nematophila colonization of symbiotic host nematodes.
Article in Molecular microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Comparative metagenomic analysis reveals the adaptive evolutionary traits of siboglinid tubeworm symbionts.Frontiers in microbiology · 2025Article
- Analyses of Xenorhabdus griffiniae genomes reveal two distinct sub-species that display intra-species variation due to prophages.BMC genomics · 2024Article
- Identification and characterization of two CRISPR/Cas systems associated with the mosquito microbiome.Access microbiology · 2023Article
- Microbiota Influences Fitness and Timing of Reproduction in the Fruit Fly Drosophila melanogaster.Microbiology spectrum · 2021Article
- Shining light on a deep-sea bacterial symbiont population structure with CRISPR.Microbial genomics · 2021Article
- The great potential of entomopathogenic bacteria Xenorhabdus and Photorhabdus for mosquito control: a review.Parasites & vectors · 2020Review
- Molecular Regulators of Entomopathogenic Nematode-Bacterial Symbiosis.Results and problems in cell differentiation · 2020Review
- A Simple Criterion for Inferring CRISPR Array Direction.Frontiers in microbiology · 2019Article
- Article
- The insect pathogenic bacterium Xenorhabdus innexi has attenuated virulence in multiple insect model hosts yet encodes a potent mosquitocidal toxin.BMC genomics · 2017Article
- Overview of CRISPR-Cas9 Biology.Cold Spring Harbor protocols · 2016Article
- Stress as a Normal Cue in the Symbiotic Environment.Trends in microbiology · 2016Review
- I can see CRISPR now, even when phage are gone: a view on alternative CRISPR-Cas functions from the prokaryotic envelope.Current opinion in infectious diseases · 2015Review
- Nuclease activity of Legionella pneumophila Cas2 promotes intracellular infection of amoebal host cells.Infection and immunity · 2015Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The bacterium Xenorhabdus nematophila is a mutualist of entomopathogenic Steinernema carpocapsae nematodes and facilitates infection of insect hosts. X. nematophila colonizes the intestine of S. carpocapsae which carries it between insects. In the X. nematophila colonization-defective mutant nilD6::Tn5, the transposon is inserted in a region lacking obvious coding potential. We demonstrate that the transposon disrupts expression of a single CRISPR RNA, NilD RNA. A variant NilD RNA also is expressed by X. nematophila strains from S. anatoliense and S. websteri nematodes. Only nilD from the S. carpocapsae strain of X. nematophila rescued the colonization defect of the nilD6::Tn5 mutant, and this mutant was defective in colonizing all three nematode host species. NilD expression depends on the presence of the associated Cas6e but not Cas3, components of the Type I-E CRISPR-associated machinery. While cas6e deletion in the complemented strain abolished nematode colonization, its disruption in the wild-type parent did not. Likewise, nilD deletion in the parental strain did not impact colonization of the nematode, revealing that the requirement for NilD is evident only in certain genetic backgrounds. Our data demonstrate that NilD RNA is conditionally necessary for mutualistic host colonization and suggest that it functions to regulate endogenous gene expression.
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