ArticleThe FEBS journal2014
A rapid and rational approach to generating isomorphous heavy-atom phasing derivatives.
Article in The FEBS journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 12 citations in OpenAlex.
- Article
- Heavy Atom Detergent/Lipid Combined X-ray Crystallography for Elucidating the Structure-Function Relationships of Membrane Proteins.Membranes · 2021Review
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- An overview of heavy-atom derivatization of protein crystals.Acta crystallographica. Section D, Structural biology · 2016Review
- Introduction: celebrating the international year of crystallography: introduction.The FEBS journal · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In attempts to replace the conventional trial-and-error heavy-atom derivative search method with a rational approach, we previously defined heavy metal compound reactivity against peptide ligands. Here, we assembled a composite pH- and buffer-dependent peptide reactivity profile for each heavy metal compound to guide rational heavy-atom derivative search. When knowledge of the best-reacting heavy-atom compound is combined with mass spectrometry assisted derivatization, and with a quick-soak method to optimize phasing, it is likely that the traditional heavy-atom compounds could meet the demand of modern high-throughput X-ray crystallography. As an example, we applied this rational heavy-atom phasing approach to determine a previously unknown mouse serum amyloid A2 crystal structure.
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