ArticleBritish journal of pharmacology2014
Identification of novel insulin mimetic drugs by quantitative total internal reflection fluorescence (TIRF) microscopy.
Article in British journal of pharmacology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Lactate-activated GPR81/FARP1 signaling drives insulin-independent glucose uptake and metabolic control.Cell research · 2026Article
- Metabolomics-driven elucidation of the synergistic therapeutic mechanism of a novel SGLT-2/PPAR-γ dual receptor supramolecular system for treatment diabetes and obesity.Journal of pharmaceutical analysis · 2025Article
- Identification of Plant Phenolics fromJournal of agricultural and food chemistry · 2025Article
- Insulin-Mimetic Activity of Herbal Extracts Identified with Large-Scale Total Internal Reflection Fluorescence Microscopy.Nutrients · 2024Article
- A comprehensiveFrontiers in pharmacology · 2024Review
- Lipoprotein Particles as Shuttles for Hydrophilic Cargo.Membranes · 2023Article
- Identification of Insulin-Mimetic Plant Extracts: From an In Vitro High-Content Screen to Blood Glucose Reduction in Live Animals.Molecules (Basel, Switzerland) · 2021Article
- Avens Root (Frontiers in pharmacology · 2021Article
- Fluorescence Microscopy-Based Quantitation of GLUT4 Translocation: High Throughput or High Content?International journal of molecular sciences · 2020Article
- Super-Resolution Live Cell Microscopy of Membrane-Proximal Fluorophores.International journal of molecular sciences · 2020Article
- Article
- Hypolipidemic effects of herbal extracts by reduction of adipocyte differentiation, intracellular neutral lipid content, lipolysis, fatty acid exchange and lipid droplet motility.Scientific reports · 2019Article
- A high-throughput chemical-genetics screen in murine adipocytes identifies insulin-regulatory pathways.The Journal of biological chemistry · 2019Article
- Insulin Mimetic Properties of Extracts Prepared fromMolecules (Basel, Switzerland) · 2018Article
- In Vitro and In Vivo Inhibition of Intestinal Glucose Transport by Guava (Psidium Guajava) Extracts.Molecular nutrition & food research · 2018Article
- Isolation of state-dependent monoclonal antibodies against the 12-transmembrane domain glucose transporter 4 using virus-like particles.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- An In Ovo Model for Testing Insulin-mimetic Compounds.Journal of visualized experiments : JoVE · 2018Article
- Gluc-HET, a complementary chick embryo model for the characterization of antidiabetic compounds.PloS one · 2017Article
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- Review
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background and purposeInsulin stimulates the transport of glucose in target tissues by triggering the translocation of glucose transporter 4 (GLUT4) to the plasma membrane. Resistance to insulin, the major abnormality in type 2 diabetes, results in a decreased GLUT4 translocation efficiency. Thus, special attention is being paid to search for compounds that are able to enhance this translocation process in the absence of insulin. EXPERIMENTAL APPROACH: Total internal reflection fluorescence (TIRF) microscopy was applied to quantify GLUT4 translocation in highly insulin-sensitive CHO-K1 cells expressing a GLUT4-myc-GFP fusion protein. KEY
resultsUsing our approach, we demonstrated GLUT4 translocation modulatory properties of selected substances and identified novel potential insulin mimetics. An increase in the TIRF signal was found to correlate with an elevated glucose uptake. Variations in the expression level of the human insulin receptor (hInsR) showed that the insulin mimetics identified stimulate GLUT4 translocation by a mechanism that is independent of the presence of the hInsR. CONCLUSIONS AND IMPLICATIONS: Taken together, the results indicate that TIRF microscopy is an excellent tool for the quantification of GLUT4 translocation and for identifying insulin mimetic drugs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.