ArticleNature nanotechnology2014
Optical trapping of individual human immunodeficiency viruses in culture fluid reveals heterogeneity with single-molecule resolution.
Article in Nature nanotechnology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed.
- Engineering Low-Power Cavity-Coupled Plasmonic Nanotweezers.Nanophotonics (Berlin, Germany) · 2026Article
- Plasmonic Optical Tweezers and Surface-Enhanced Raman Spectroscopy: Fundamentals, Single-Entity Applications, and the Evolving Role of Artificial Intelligence.Bioengineering (Basel, Switzerland) · 2026Review
- Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.European biophysics journal : EBJ · 2026Review
- Virus-like antigen display delivers a stand-alone danger signal through the BCR that circumvents tolerance.bioRxiv : the preprint server for biology · 2026Article
- Flexible, stretchable, on-chip optical tweezers for high-throughput bioparticle manipulation.Light, science & applications · 2026Article
- Article
- Optical sorting: past, present and future.Light, science & applications · 2025Review
- Analyzing the factors affecting virus invasion by quantitative single-particle analysis.Virulence · 2024Article
- Optical tweezers in biomedical research - progress and techniques.Journal of medicine and life · 2024Review
- An Integrated Signaling Threshold Initiates IgG Response toward Virus-like Immunogens.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- Optical tweezing of microparticles and cells using silicon-photonics-based optical phased arrays.Nature communications · 2024Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Deep learning-based size prediction for optical trapped nanoparticles and extracellular vesicles from limited bandwidth camera detection.Biomedical optics express · 2024Article
- Molecular basis for potent B cell responses to antigen displayed on particles of viral size.Nature immunology · 2023Article
- Article
- Optofluidic Tweezers: Efficient and Versatile Micro/Nano-Manipulation Tools.Micromachines · 2023Review
- Viro-fluidics: Real-time analysis of virus production kinetics at the single-cell level.Biophysical reports · 2022Article
- Nanogap Electrode-Enabled Versatile Electrokinetic Manipulation of Nanometric Species in Fluids.Biosensors · 2022Article
- Structural characterization of β-propiolactone inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) particles.Microscopy research and technique · 2022Article
- Optical Pulling Using Chiral Metalens as a Photonic Probe.Nanomaterials (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Optical tweezers use the momentum of photons to trap and manipulate microscopic objects, contact-free, in three dimensions. Although this technique has been widely used in biology and nanotechnology to study molecular motors, biopolymers and nanostructures, its application to study viruses has been very limited, largely due to their small size. Here, using optical tweezers that can simultaneously resolve two-photon fluorescence at the single-molecule level, we show that individual HIV-1 viruses can be optically trapped and manipulated, allowing multi-parameter analysis of single virions in culture fluid under native conditions. We show that individual HIV-1 differs in the numbers of envelope glycoproteins by more than one order of magnitude, which implies substantial heterogeneity of these virions in transmission and infection at the single-particle level. Analogous to flow cytometry for cells, this fluid-based technique may allow ultrasensitive detection, multi-parameter analysis and sorting of viruses and other nanoparticles in biological fluid with single-molecule resolution.
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Registered trials
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