ArticleBiochimie2014
Bound cardiolipin is essential for cytochrome c oxidase proton translocation.
Article in Biochimie, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Effects of Cannabidiol on TAFAZZIN-Deficient B-Lymphoblastoid Cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- Electron Paramagnetic Resonance Spectroscopy of Spin-Labeled Lipids for Studying Lipid-Protein Interaction in Model and Biomembranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The role of cardiolipin in mitochondrial dynamics and quality control in neuronal ischemia/reperfusion injury.Cell death & disease · 2025Review
- Hepatic retinol dehydrogenase 11 dampens stress associated with the maintenance of cellular cholesterol levels.Molecular metabolism · 2024Article
- Interaction of Terminal Oxidases with Amphipathic Molecules.International journal of molecular sciences · 2023Review
- Review
- Stressed to death: Mitochondrial stress responses connect respiration and apoptosis in cancer.Molecular cell · 2022Review
- Structural basis of mammalian complex IV inhibition by steroids.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Review
- Monomeric structure of an active form of bovine cytochromeProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- Tetra-linoleoyl cardiolipin depletion plays a major role in the pathogenesis of sarcopenia.Medical hypotheses · 2019Article
- The folding, stability and function of lactose permease differ in their dependence on bilayer lipid composition.Scientific reports · 2017Article
- Dimer interface of bovine cytochrome c oxidase is influenced by local posttranslational modifications and lipid binding.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Delipidation of cytochrome c oxidase from Rhodobacter sphaeroides destabilizes its quaternary structure.Biochimie · 2016Article
- Mitochondrial cytochrome c oxidase deficiency.Clinical science (London, England : 1979) · 2016Review
- The kinetic stability of cytochrome C oxidase: effect of bound phospholipid and dimerization.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The proton pumping activity of bovine heart cytochrome c oxidase (CcO) is completely inhibited when all of the cardiolipin (CL) is removed from the enzyme to produce monomeric CcO containing only 11 subunits. Only dimeric enzyme containing all 13 subunits and 2-4 cardiolipin per CcO monomer exhibits a "normal" proton translocating stoichiometry of ∼1.0 H(+) per/e(-) when reconstituted into phospholipid vesicles. These fully active proteoliposomes have high respiratory control ratios (RCR = 7-15) with 75-85% of the CcO oriented with the cytochrome c binding sites exposed to the external medium. In contrast, reconstitution of CL-free CcO results in low respiratory control ratios (RCR < 5) with the enzyme randomly oriented in the vesicles, i.e., ∼50 percent oriented with the cytochrome c binding site exposed on the outside of the vesicle. Addition of exogenous CL to the CL-free enzyme completely restores electron transport activity, but restoration of proton pumping activity does not occur. This is true whether CL is added to CL-free CcO prior to reconstitution into phospholipid vesicles, or whether CL is included in the phospholipid mixture that is used to form the vesicles. Another consequence of CL removal is the inability of the 11-subunit, CL-free enzyme to dimerize upon exposure to either cholate or the cholate/PC/PE/CL mixture used during proteoliposome formation (monomeric, 13-subunit, CL-containing CcO completely dimerizes under these conditions). Therefore, a major difference between reconstitution of CL-free and CL-containing CcO is the incorporation of monomeric, rather than dimeric CcO into the vesicles. We conclude that bound CL is necessary for proper insertion of CcO into phospholipid vesicles and normal proton translocation.
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