ArticleThe Journal of infectious diseases2014
The presence of the pAA plasmid in the German O104:H4 Shiga toxin type 2a (Stx2a)-producing enteroaggregative Escherichia coli strain promotes the translocation of Stx2a across an epithelial cell monolayer.
Article in The Journal of infectious diseases, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 38 citations in OpenAlex.
- Linking land use to the likely origins of third-generation cephalosporin-resistant Enterobacterales in freshwater.Applied and environmental microbiology · 2026Article
- EnteroaggregativeEcoSal Plus · 2025Review
- Lipoprotein Lpp and L, D-transpeptidases regulate the master regulator of virulence AggR in EAEC.Scientific reports · 2025Article
- A Comprehensive Review on Shiga Toxin Subtypes and Their Niche-Related Distribution Characteristics in Shiga-Toxin-ProducingMicroorganisms · 2024Review
- Shiga-Toxin-Producing Strains ofMicroorganisms · 2023Article
- EnterohemorrhagicInternational journal of molecular sciences · 2022Review
- Article
- Integrating evolutionary aspects into dual-use discussion: the cases of influenza virus and enterohemorrhagicEvolution, medicine, and public health · 2021Review
- Enteroaggregative E. coli Adherence to Human Heparan Sulfate Proteoglycans Drives Segment and Host Specific Responses to Infection.PLoS pathogens · 2020Article
- Redefining enteroaggregative Escherichia coli (EAEC): Genomic characterization of epidemiological EAEC strains.PLoS neglected tropical diseases · 2020Article
- Global distribution of epidemic-related Shiga toxin 2 encoding phages among enteroaggregative Escherichia coli.Scientific reports · 2020Article
- QseC Signaling in the Outbreak O104:H4Journal of bacteriology · 2019Article
- The Role of the AggR Regulon in the Virulence of the Shiga Toxin-Producing EnteroaggregativeFrontiers in microbiology · 2019Article
- Host-specific differences in the contribution of an ESBL IncI1 plasmid to intestinal colonization by Escherichia coli O104:H4.The Journal of antimicrobial chemotherapy · 2018Article
- Article
- Flavonoid-derived anisotropic silver nanoparticles inhibit growth and change the expression of virulence genes inRSC advances · 2018Article
- EnteroaggregativemBio · 2017Article
- The primary transcriptome of the Escherichia coli O104:H4 pAA plasmid and novel insights into its virulence gene expression and regulation.Scientific reports · 2016Article
- Polysorbates prevent biofilm formation and pathogenesis of Escherichia coli O104:H4.Biofouling · 2016Article
- EnterohemorrhagicCellular and molecular gastroenterology and hepatology · 2016Article
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8 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundA Shiga toxin type 2a (Stx2a)-producing enteroaggregative Escherichia coli (EAEC) strain of serotype O104:H4 caused a large outbreak in 2011 in northern Europe. Pathogenic mechanisms for this strain are unclear. We hypothesized that EAEC genes encoded on the pAA virulence plasmid promoted the translocation of Stx2a across the intestinal mucosa.
methodsWe investigated the potential contribution of pAA by using mutants of Stx-EAEC strain C227-11, either cured of the pAA plasmid or deleted for individual known pAA-encoded virulence genes (ie, aggR, aggA, and sepA). The resulting mutants were tested for their ability to induce interleukin 8 (IL-8) secretion and translocation of Stx2a across a polarized colonic epithelial (T84 cell) monolayer.
resultsWe found that deletion of aggR or aggA significantly reduced bacterial adherence and (independently) translocation of Stx2a across the T84-cell monolayer. Moreover, deletion of aggR, aggA, sepA, or the Stx2a-encoding phage from C227-11 resulted in reduced secretion of IL-8 from the infected monolayer.
conclusionsOur data suggest that the AggR-regulated aggregative adherence fimbriae I enhance inflammation and enable the outbreak strain to both adhere to epithelial cells and translocate Stx2a across the intestinal epithelium.
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