Evidence map›Paper›PMID 25038247›Full record

ArticleStem cells translational medicine2014

Testicular niche required for human spermatogonial stem cell expansion.

James F Smith, Pamela Yango, Eran Altman, Shweta Choudhry, Andrea Poelzl, Alberuni M Zamah, Mitchell Rosen, Peter C Klatsky, Nam D Tran

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
8.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 75 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Perspectives: Methods for Evaluating Primate Spermatogonial Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  5. Article
  6. Review
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  11. Review
  12. Article
  13. Article
  14. Spermatogonial Stem Cell Culture in Oncofertility.The Urologic clinics of North America · 2020
    Review
  15. Article
  16. Review
  17. Article
  18. Article
  19. Role of stem cells in fertility preservation: current insights.Stem cells and cloning : advances and applications · 2019
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

James F SmithDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Pamela YangoDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Eran AltmanDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Shweta ChoudhryDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Andrea PoelzlDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Alberuni M ZamahDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Mitchell RosenDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Peter C KlatskyDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA.
Nam D TranDepartments of Urology and Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Francisco, San Francisco, California, USA; Helen Schneider Hospital for Women, Rabin Medical Center, Petah-Tiqva, Israel; Department of Obstetrics and Gynecology, Albert Einstein University, Bronx, New York, USA trann@obgyn.ucsf.edu.
University of California, San Francisco · USAlbert Einstein College of Medicine · USRabin Medical Center · IL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prepubertal boys treated with high-dose chemotherapy do not have an established means of fertility preservation because no established in vitro technique exists to expand and mature purified spermatogonial stem cells (SSCs) to functional sperm in humans. In this study, we define and characterize the unique testicular cellular niche required for SSC expansion using testicular tissues from men with normal spermatogenesis. Highly purified SSCs and testicular somatic cells were isolated by fluorescence-activated cell sorting using SSEA-4 and THY1 as markers of SSCs and somatic cells. Cells were cultured on various established niches to assess their role in SSC expansion in a defined somatic cellular niche. Of all the niches examined, cells in the SSEA-4 population exclusively bound to adult testicular stromal cells, established colonies, and expanded. Further characterization of these testicular stromal cells revealed distinct mesenchymal markers and the ability to undergo differentiation along the mesenchymal lineage, supporting a testicular multipotent stromal cell origin. In vitro human SSC expansion requires a unique niche provided exclusively by testicular multipotent stromal cells with mesenchymal properties. These findings provide an important foundation for developing methods of inducing SSC growth and maturation in prepubertal testicular tissue, essential to enabling fertility preservation for these boys.

Indexed as

AdultAdult Stem CellsCell Culture TechniquesCell DifferentiationCell SeparationFertility PreservationFlow CytometryHumansMaleMesenchymal Stem CellsMicroscopy, ConfocalSpermatogenesisSpermatozoaStem Cell NicheTestisCancerCell surface markersClinical translationMesenchymal stem cellsSpermatogonial stem cells

Identifiers

PMID25038247
PMCPMC4149303
OpenAlexW2111078095

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.