ReviewApplied and environmental microbiology2014
Dead or alive: molecular assessment of microbial viability.
Review in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers, 3 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
141 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- AI-Enabled Imaging for Pathogen Detection Under Stress Conditions: A Systematic Review.Comprehensive reviews in food science and food safety · 2026Pooled it
- Oral microbiome and lesion-associated microbiological findings in medication-related osteonecrosis of the jaw: a systematic review and evidence map.Frontiers in cellular and infection microbiology · 2026Pooled it
- Epidemiological, Radiographical, and Laboratorial Characteristics of Chinese Asymptomatic Cases With COVID-19: A Systematic Review and Meta-Analysis.Frontiers in public health · 2022Pooled it
- Spontaneous clearance of asymptomatic anogenital and pharyngealSexually transmitted infections · 2023Trial
- Trial
- Article
- Establishment of methods for the detection ofVeterinary and animal science · 2026Article
- Discriminating Between Living and Dead Bacteria With a Field-Effect Transistor Based on Nano-Channels.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Emerging Microbiological and Sensor-Based Approaches for Biofilm Detection in Meat and Poultry Processing Environments.Foods (Basel, Switzerland) · 2026Review
- Identifying viableMicrobiology spectrum · 2026Article
- Advances in Detecting Viable/Dead Foodborne Microorganisms Using Diverse Functional Nucleic Acid-Based Molecular Recognition.Biosensors · 2026Review
- Altered distribution of chronicity-deficientMicrobiology (Reading, England) · 2026Article
- Integrated MALDI-TOF MS, Microbiological, Physicochemical and Sensory Assessment of Spoilage in Vacuum-Packaged Chicken Breast During Refrigerated Storage.Foods (Basel, Switzerland) · 2026Article
- Comparative evaluation of viability PCR reagents highlights the superior efficacy of PMAxx azo dye for bacterial and viral viability discrimination using real-time and digital PCR.Microbiology spectrum · 2026Article
- Molecular Monitoring in Soil Bioremediation: From Genetic Potential to Verified Pathway Operation.International journal of molecular sciences · 2026Review
- Infant gut microbiota coverage by different culture media.BMC microbiology · 2026Article
- Towards precision management ofFrontiers in cellular and infection microbiology · 2026Article
- Simultaneous measurement of 16S-rRNA and pre-16S-rRNA as a strategy to monitor clinical tuberculosis.Frontiers in antibiotics · 2026Article
- Multi-dimensional dynamic assessment revealed the antibiotic-induced pseudo-germ-free model in SD rats stabilized within 4 days.Frontiers in microbiology · 2026Article
- Novel molecular assessment method using precursor ribosomal RNA to rapidly quantify hard-to-monitor pathogens in aqueous suspensions: application to ultraviolet disinfection ofJournal of water and health · 2025Article
81 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleic acid-based analytical methods, ranging from species-targeted PCRs to metagenomics, have greatly expanded our understanding of microbiological diversity in natural samples. However, these methods provide only limited information on the activities and physiological states of microorganisms in samples. Even the most fundamental physiological state, viability, cannot be assessed cross-sectionally by standard DNA-targeted methods such as PCR. New PCR-based strategies, collectively called molecular viability analyses, have been developed that differentiate nucleic acids associated with viable cells from those associated with inactivated cells. In order to maximize the utility of these methods and to correctly interpret results, it is necessary to consider the physiological diversity of life and death in the microbial world. This article reviews molecular viability analysis in that context and discusses future opportunities for these strategies in genetic, metagenomic, and single-cell microbiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.