ArticleThe Journal of biological chemistry2014
Dynamics of the heme-binding bacterial gas-sensing dissimilative nitrate respiration regulator (DNR) and activation barriers for ligand binding and escape.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Engineering a highly selective, hemoprotein-based scavenger as a carbon monoxide poisoning antidote with no hypertensive effect.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- H-NOX Influences Biofilm Formation, Central Metabolism, and Quorum Sensing inJournal of proteome research · 2024Article
- Tyrosol blocks E. coli anaerobic biofilm formation via YbfA and FNR to increase antibiotic susceptibility.Nature communications · 2024Article
- The circularly permuted globin domain of androglobin exhibits atypical heme stabilization and nitric oxide interaction.Chemical science · 2024Article
- Interrelation between Stress Management and Secretion Systems ofPathogens (Basel, Switzerland) · 2022Article
- Molecular insights into the role of heme in the transcriptional regulatory system AppA/PpsR.Biophysical journal · 2022Article
- Early processes in heme-based CO-sensing proteins.Frontiers in molecular biosciences · 2022Review
- The C-terminal tail extension of myosin 16 acts as a molten globule, including intrinsically disordered regions, and interacts with the N-terminal ankyrin.The Journal of biological chemistry · 2021Article
- Nitrosative stress sensing in Porphyromonas gingivalis: structure of and heme binding by the transcriptional regulator HcpR.Acta crystallographica. Section D, Structural biology · 2019Article
- Bacterial Heme-Based Sensors of Nitric Oxide.Antioxidants & redox signaling · 2018Review
- Ultrafast CO Kinetics in Heme Proteins: Adiabatic Ligand Binding and Heavy Atom Tunneling.Journal of the American Chemical Society · 2017Article
- Heme and nitric oxide binding by the transcriptional regulator DnrF from the marine bacteriumThe Journal of biological chemistry · 2017Article
- Ultrafast photochemistry of the bcPhysical chemistry chemical physics : PCCP · 2017Article
- Bacterial Stress Responses during Host Infection.Cell host & microbe · 2016Review
- Discrimination and Integration of Stress Signals by Pathogenic Bacteria.Cell host & microbe · 2016Review
- Determining Roles of Accessory Genes in Denitrification by Mutant Fitness Analyses.Applied and environmental microbiology · 2016Article
- Origin and Impact of Nitric Oxide in Pseudomonas aeruginosa Biofilms.Journal of bacteriology · 2016Review
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNR (dissimilative nitrate respiration regulator) is a heme-binding transcription factor that is involved in the regulation of denitrification in Pseudomonas aeruginosa. In the ferrous deoxy state, the heme is 6-coordinate; external NO and CO can replace an internal ligand. Using fluorescence anisotropy, we show that high-affinity sequence-specific DNA binding occurs only when the heme is nitrosylated, consistent with the proposed function of DNR as NO sensor and transcriptional activator. This role is moreover supported by the NO "trapping" properties revealed by ultrafast spectroscopy that are similar to those of other heme-based NO sensor proteins. Dissociated CO-heme pairs rebind in an essentially barrierless way. This process competes with migration out of the heme pocket. The latter process is thermally activated (Ea ∼ 7 kJ/mol). This result is compared with other heme proteins, including the homologous CO sensor/transcription factor CooA, variants of the 5-coordinate mycobacterial sensor DosT and the electron transfer protein cytochrome c. This comparison indicates that thermal activation of ligand escape from the heme pocket is specific for systems where an external ligand replaces an internal one. The origin of this finding and possible implications are discussed.
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