ArticlePlant physiology2014
Tandem amplification of a chromosomal segment harboring 5-enolpyruvylshikimate-3-phosphate synthase locus confers glyphosate resistance in Kochia scoparia.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 131 citations in OpenAlex.
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- Widespread non-target-site resistance in Setaria viridis to four classes of herbicide.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Evolution of Highly Repetitive Silk Genes in the Luna Moth, Actias luna.Genome biology and evolution · 2026Article
- The genetic architecture and spatiotemporal dynamics of adaptation across human-modified landscapes.The New phytologist · 2026Review
- Novel amino acid substitutions in Protoporphyrinogen oxidase 1 endow resistance to PPO-inhibiting herbicides inFrontiers in plant science · 2026Article
- FHY3/FAR1 transposable elements generate adaptive genetic variation in the Bassia scoparia genome.Pest management science · 2025Article
- Over-expression and increased copy numbers of a cytochrome P450 and two UDP-glucuronosyltransferase genes in macrocyclic lactone resistant Psoroptes ovis of cattle.PLoS pathogens · 2025Article
- Appearance of transient heteromorphic large chromosome in glyphosate-resistant Amaranthus tuberculatus.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- A rearranged Amaranthus palmeri extrachromosomal circular DNA confers resistance to glyphosate and glufosinate.The Plant cell · 2025Article
- Current status of community resources and priorities for weed genomics research.Genome biology · 2024Review
- Paralog transcriptional differentiation in the D. melanogaster-specific gene family Sdic across populations and spermatogenesis stages.Communications biology · 2023Article
- Subtelomeric 5-enolpyruvylshikimate-3-phosphate synthase copy number variation confers glyphosate resistance in Eleusine indica.Nature communications · 2023Article
- First report of glyphosate-resistant downy brome (Bromus tectorum L.) in Canada.Scientific reports · 2022Article
- Article
- Genomic-based epidemiology reveals independent origins and gene flow of glyphosate resistance in Bassia scoparia populations across North America.Molecular ecology · 2021Article
- Effect of EPSPS gene copy number and glyphosate selection on fitness of glyphosate-resistant Bassia scoparia in the field.Scientific reports · 2021Article
- Fitness Cost Associated With EnhancedFrontiers in plant science · 2021Article
- Evolution of Glyphosate-Resistant Weeds.Reviews of environmental contamination and toxicology · 2021Review
- High gene flow maintains genetic diversity following selection for high EPSPS copy number in the weed kochia (Amaranthaceae).Scientific reports · 2020Article
- Understanding the Early Evolutionary Stages of a Tandem Drosophilamelanogaster-Specific Gene Family: A Structural and Functional Population Study.Molecular biology and evolution · 2020Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent rapid evolution and spread of resistance to the most extensively used herbicide, glyphosate, is a major threat to global crop production. Genetic mechanisms by which weeds evolve resistance to herbicides largely determine the level of resistance and the rate of evolution of resistance. In a previous study, we determined that glyphosate resistance in Kochia scoparia is due to the amplification of the 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) gene, the enzyme target of glyphosate. Here, we investigated the genomic organization of the amplified EPSPS copies using fluorescence in situ hybridization (FISH) and extended DNA fiber (Fiber FISH) on K. scoparia chromosomes. In both glyphosate-resistant K. scoparia populations tested (GR1 and GR2), FISH results displayed a single and prominent hybridization site of the EPSPS gene localized on the distal end of one pair of homologous metaphase chromosomes compared with a faint hybridization site in glyphosate-susceptible samples (GS1 and GS2). Fiber FISH displayed 10 copies of the EPSPS gene (approximately 5 kb) arranged in tandem configuration approximately 40 to 70 kb apart, with one copy in an inverted orientation in GR2. In agreement with FISH results, segregation of EPSPS copies followed single-locus inheritance in GR1 population. This is the first report of tandem target gene amplification conferring field-evolved herbicide resistance in weed populations.
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