ArticlePlant physiology2014
CPK13, a noncanonical Ca2+-dependent protein kinase, specifically inhibits KAT2 and KAT1 shaker K+ channels and reduces stomatal opening.
Article in Plant physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 114 citations in OpenAlex.
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- OsCPK12 phosphorylates OsCATA and OsCATC to regulate HPlant communications · 2024Article
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- The Molecular Mechanism of Potassium Absorption, Transport, and Utilization in Rice.International journal of molecular sciences · 2023Review
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- The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata.The Plant cell · 2022Article
- The role of CDPKs in plant development, nutrient and stress signaling.Frontiers in genetics · 2022Review
- Constitutive Active CPK30 Interferes With Root Growth and Endomembrane Trafficking inFrontiers in plant science · 2022Article
- Genome Editing Targets for Improving Nutrient Use Efficiency and Nutrient Stress Adaptation.Frontiers in genetics · 2022Review
- Structural and Functional Insights into the Role of Guard Cell Ion Channels in Abiotic Stress-Induced Stomatal Closure.Plants (Basel, Switzerland) · 2021Review
- Molecular Evolution of Calcium Signaling and Transport in Plant Adaptation to Abiotic Stress.International journal of molecular sciences · 2021Review
- Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.The Plant cell · 2021Article
- CsCDPK6, a CsSAMS1-Interacting Protein, Affects Polyamine/Ethylene Biosynthesis in Cucumber and Enhances Salt Tolerance by Overexpression in Tobacco.International journal of molecular sciences · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca(2) (+)-dependent protein kinases (CPKs) form a large family of 34 genes in Arabidopsis (Arabidopsis thaliana). Based on their dependence on Ca(2+), CPKs can be sorted into three types: strictly Ca(2+)-dependent CPKs, Ca(2+)-stimulated CPKs (with a significant basal activity in the absence of Ca(2+)), and essentially calcium-insensitive CPKs. Here, we report on the third type of CPK, CPK13, which is expressed in guard cells but whose role is still unknown. We confirm the expression of CPK13 in Arabidopsis guard cells, and we show that its overexpression inhibits light-induced stomatal opening. We combine several approaches to identify a guard cell-expressed target. We provide evidence that CPK13 (1) specifically phosphorylates peptide arrays featuring Arabidopsis K(+) Channel KAT2 and KAT1 polypeptides, (2) inhibits KAT2 and/or KAT1 when expressed in Xenopus laevis oocytes, and (3) closely interacts in plant cells with KAT2 channels (Förster resonance energy transfer-fluorescence lifetime imaging microscopy). We propose that CPK13 reduces stomatal aperture through its inhibition of the guard cell-expressed KAT2 and KAT1 channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.