ArticleThe ISME journal2014
Exploring the functional soil-microbe interface and exoenzymes through soil metaexoproteomics.
Article in The ISME journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- Extraction of Proteins from Soil.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Stimulation of Distinct Rhizosphere Bacteria Drives Phosphorus and Nitrogen Mineralization in Oilseed Rape under Field Conditions.mSystems · 2022Article
- New opportunities in plant microbiome engineering for increasing agricultural sustainability under stressful conditions.Frontiers in plant science · 2022Review
- Removal of primary nutrient degraders reduces growth of soil microbial communities with genomic redundancy.Frontiers in microbiology · 2022Article
- Increasing the power of interpretation for soil metaproteomics data.Microbiome · 2021Article
- Recent advances in the bioprospection and applications of chitinolytic bacteria for valorization of waste chitin.Archives of microbiology · 2021Review
- 100 Days of marine Synechococcus-Ruegeria pomeroyi interaction: A detailed analysis of the exoproteome.Environmental microbiology · 2018Article
- Defining functional diversity for lignocellulose degradation in a microbial community using multi-omics studies.Biotechnology for biofuels · 2018Article
- Enhanced Growth and Activities of the Dominant Functional Microbiota of Chicken Manure Composts in the Presence of Maize Straw.Frontiers in microbiology · 2018Article
- Challenges and Approaches in Microbiome Research: From Fundamental to Applied.Frontiers in plant science · 2018Review
- Revealing the insoluble metasecretome of lignocellulose-degrading microbial communities.Scientific reports · 2017Article
- Complex N acquisition by soil diazotrophs: how the ability to release exoenzymes affects N fixation by terrestrial free-living diazotrophs.The ISME journal · 2017Article
- Comparative genomic, proteomic and exoproteomic analyses of three Pseudomonas strains reveals novel insights into the phosphorus scavenging capabilities of soil bacteria.Environmental microbiology · 2016Article
- Soil restoration with organic amendments: linking cellular functionality and ecosystem processes.Scientific reports · 2015Article
- Functional distinctness in the exoproteomes of marine Synechococcus.Environmental microbiology · 2015Article
- Designing and Implementing an Assay for the Detection of Rare and Divergent NRPS and PKS Clones in European, Antarctic and Cuban Soils.PloS one · 2015Article
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functionally important proteins at the interface of cell and soil are of potentially low abundance when compared with commonly recovered intracellular proteins. A novel approach was developed and used to extract the metaexoproteome, the subset of proteins found outside the cell, in the context of a soil enriched with the nitrogen-containing recalcitrant polymer chitin. The majority of proteins recovered was of bacterial origin and localized to the outer membrane or extracellular milieu. A wide variety of transporter proteins were identified, particularly those associated with amino-acid and phosphate uptake. The metaexoproteome extract retained chitinolytic activity and we were successful in detecting Nocardiopsis-like chitinases that correlated with the glycoside hydrolase family 18 (GH18) chi gene data and metataxonomic analysis. Nocardiopsis-like chitinases appeared to be solely responsible for chitinolytic activity in soil. This is the first study to detect and sequence bacterial exoenzymes with proven activity in the soil enzyme pool.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.