ArticlePloS one2014
Production of the Escherichia coli common pilus by uropathogenic E. coli is associated with adherence to HeLa and HTB-4 cells and invasion of mouse bladder urothelium.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 48 citations in OpenAlex.
- Human body temperature cues widespread changes in virulence gene expression in uropathogenicInfection and immunity · 2026Article
- Trained Immunity in Bladder ILC3s Enhances Mucosal Defense Against Recurrent Urinary Tract Infections.Biomedicines · 2025Article
- The Nucleoid-Associated Protein Fis Represses Type 3 Fimbriae to Modulate Biofilm and Adherence Formation inMicroorganisms · 2025Article
- An Atypical Kappa-Class Chaperone-Usher Fimbriae of a Human Enterotoxigenic Escherichia coli Strain Shows Multi-Host Adherence and Distinct Phylogenetic Feature.Microbiology and immunology · 2025Article
- Comprehensive Genomic Analysis of UropathogenicPathogens (Basel, Switzerland) · 2024Article
- Genotypic and phenotypic characterisation of asymptomatic bacteriuria (ABU) isolates displaying bacterial interference against multi-drug resistant uropathogenic E. Coli.Archives of microbiology · 2024Article
- Chitosan-Coated Silver Nanoparticles Inhibit Adherence and Biofilm Formation of UropathogenicACS infectious diseases · 2024Article
- Urinary Tract Infections Caused by UropathogenicInternational journal of molecular sciences · 2023Review
- Comparative genomic analysis of uropathogenicFrontiers in microbiology · 2023Article
- Antimicrobial Peptide LCN2 Inhibited UropathogenicInternational journal of molecular sciences · 2022Article
- Lack of Direct Correlation between Biofilm Formation and Antimicrobial Resistance in ClinicalMicroorganisms · 2022Article
- Genome placement of alpha-haemolysin cluster is associated with alpha-haemolysin sequence variation, adhesin and iron acquisition factor profile ofMicrobial genomics · 2021Article
- Virulence Potential of a Multidrug-ResistantMicroorganisms · 2020Article
- Article
- The Interaction ofFrontiers in cellular and infection microbiology · 2019Article
- Molecular Epidemiology of Extraintestinal PathogenicEcoSal Plus · 2018Review
- Distribution of Major Pilin Subunit Genes Among Atypical EnteropathogenicFrontiers in microbiology · 2018Article
- Article
- Evaluation of Recombinant AttenuatedFrontiers in immunology · 2017Article
- Invasion of Host Cells and Tissues by Uropathogenic Bacteria.Microbiology spectrum · 2016Review
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Uropathogenic Escherichia coli (UPEC) strains cause urinary tract infections and employ type 1 and P pili in colonization of the bladder and kidney, respectively. Most intestinal and extra-intestinal E. coli strains produce a pilus called E. coli common pilus (ECP) involved in cell adherence and biofilm formation. However, the contribution of ECP to the interaction of UPEC with uroepithelial cells remains to be elucidated. Here, we report that prototypic UPEC strains CFT073 and F11 mutated in the major pilin structural gene ecpA are significantly deficient in adherence to cultured HeLa (cervix) and HTB-4 (bladder) epithelial cells in vitro as compared to their parental strains. Complementation of the ecpA mutant restored adherence to wild-type levels. UPEC strains produce ECP upon growth in Luria-Bertani broth or DMEM tissue culture medium preferentially at 26°C, during incubation with cultured epithelial cells in vitro at 37°C, and upon colonization of mouse bladder urothelium ex vivo. ECP was demonstrated on and inside exfoliated bladder epithelial cells present in the urine of urinary tract infection patients. The ability of the CFT073 ecpA mutant to invade the mouse tissue was significantly reduced. The presence of ECP correlated with the architecture of the biofilms produced by UPEC strains on inert surfaces. These data suggest that ECP can potentially be produced in the bladder environment and contribute to the adhesive and invasive capabilities of UPEC during its interaction with the host bladder. We propose that along with other known adhesins, ECP plays a synergistic role in the multi-step infection of the urinary tract.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.