ArticleJournal of the American Chemical Society2014
Reconstitution of peptidoglycan cross-linking leads to improved fluorescent probes of cell wall synthesis.
Article in Journal of the American Chemical Society, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
55 citing papers in PubMed, 111 citations in OpenAlex.
- Fingerprint profiling human gut microbiota through boronic acid-based sensor array and its application on colorectal cancer classification.Nature communications · 2026Article
- Decoding the Penicillin-Binding Proteins with Activity-Based Probes.Accounts of chemical research · 2025Review
- Article
- Noninvasive Analysis of Peptidoglycan from Living Animals.Bioconjugate chemistry · 2024Article
- A Metabolic-Tag-Based Method for Assessing the Permeation of Small Molecules Across the Mycomembrane in Live Mycobacteria.Angewandte Chemie (International ed. in English) · 2023Article
- N-Amidation of Nitrogen-Containing Heterocyclic Compounds: Can We Apply Enzymatic Tools?Bioengineering (Basel, Switzerland) · 2023Review
- Real-time non-invasive fluorescence imaging of gut commensal bacteria to detect dynamic changes in the microbiome of live mice.Cell chemical biology · 2022Article
- Inhibition ofMicrobiology (Reading, England) · 2022Article
- Chemical Reporters for Bacterial Glycans: Development and Applications.Chemical reviews · 2022Review
- Impact of crossbridge structure on peptidoglycan crosslinking: A synthetic stem peptide approach.Methods in enzymology · 2022Article
- Systematic Assessment of Accessibility to the Surface ofACS chemical biology · 2021Article
- SaccuFlow: A High-Throughput Analysis Platform to Investigate Bacterial Cell Wall Interactions.ACS infectious diseases · 2021Article
- Induction of Endogenous Antibody Recruitment to the Surface of the PathogenACS infectious diseases · 2021Article
- Characterisation of a putative M23-domain containing protein in Mycobacterium tuberculosis.PloS one · 2021Article
- Facile Synthesis and Metabolic Incorporation ofACS chemical biology · 2020Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Assembly of Peptidoglycan Fragments-A Synthetic Challenge.Pharmaceuticals (Basel, Switzerland) · 2020Review
- Remodeling of Cross-bridges Controls Peptidoglycan Cross-linking Levels in Bacterial Cell Walls.ACS chemical biology · 2020Article
- Chemical tools for selective activity profiling of bacterial penicillin-binding proteins.Methods in enzymology · 2020Article
- Chemical tools to characterize peptidoglycan synthases.Current opinion in chemical biology · 2019Review
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
The peptidoglycan precursor, Lipid II, produced in the model Gram-positive bacterium Bacillus subtilis differs from Lipid II found in Gram-negative bacteria such as Escherichia coli by a single amidation on the peptide side chain. How this difference affects the cross-linking activity of penicillin-binding proteins (PBPs) that assemble peptidoglycan in cells has not been investigated because B. subtilis Lipid II was not previously available. Here we report the synthesis of B. subtilis Lipid II and its use by purified B. subtilis PBP1 and E. coli PBP1A. While enzymes from both organisms assembled B. subtilis Lipid II into glycan strands, only the B. subtilis enzyme cross-linked the strands. Furthermore, B. subtilis PBP1 catalyzed the exchange of both D-amino acids and D-amino carboxamides into nascent peptidoglycan, but the E. coli enzyme only exchanged D-amino acids. We exploited these observations to design a fluorescent D-amino carboxamide probe to label B. subtilis PG in vivo and found that this probe labels the cell wall dramatically better than existing reagents.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.