ArticlePloS one2014
Structure-based rational design to enhance the solubility and thermostability of a bacterial laccase Lac15.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 22 citations in OpenAlex.
- Structural insights into the catalytic mechanism of lovastatin hydrolase.The Journal of biological chemistry · 2020Article
- Degradation of Aflatoxin BToxins · 2019Article
- The first fungal laccase with an alkaline pH optimum obtained by directed evolution and its application in indigo dye decolorization.AMB Express · 2019Article
- Enhancement of Soluble Expression and Biochemical Characterization of Two Epoxide Hydrolases fromIranian journal of biotechnology · 2019Article
- Yeast Hosts for the Production of Recombinant Laccases: A Review.Molecular biotechnology · 2016Review
- Laccases of prokaryotic origin: enzymes at the interface of protein science and protein technology.Cellular and molecular life sciences : CMLS · 2015Review
- How to enjoy laccases.Cellular and molecular life sciences : CMLS · 2015Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
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Abstract
Bacterial laccases are ideal alternatives of fungal laccases for specific industrial applications due to specific characteristics such as alkalescence dependence and high chloride tolerance. However, some bacterial laccases presented as inclusion bodies when expressing in Escherichia coli and showed thermal instability. In this study, rational design was employed to enhance the solubility and the thermostablity of the bacterial laccase Lac15-His6 based on the crystal structure obtained previously. After deletion of His-tag and residues323-332, the obtained Lac15D was completely expressed in soluble form even at a higher temperature of 28°C, compared to only 50% of Lac15-His6 expressed solubly at 16°C. It showed a two-time higher activity at temperatures lower than 35°C and a half-life increasing from 72 min to 150 min at 45°C. When used in chromogenic reactions, Lac15D showed constant activity toward dye precursors and their combinations under alkaline conditions, demonstrating its application potential in hair coloring biotechnology.
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