ArticleACS synthetic biology2014
Modular, multi-input transcriptional logic gating with orthogonal LacI/GalR family chimeras.
Article in ACS synthetic biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Programming Next-Generation Synthetic Biosensors by Genetic Circuit Design.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Expanding the toolkit of LacI/GalR chimeras.PloS one · 2026Article
- Designing modular genetic sensors with TtgR/AcrR family transcriptional regulators by using a statistical and data-driven approach.Nucleic acids research · 2025Article
- Modular and signal-responsive transcriptional regulation using CRISPRi-aided genetic switches in Escherichia coli.Journal of biological engineering · 2025Article
- Biological Switches: Past and Future Milestones of Transcription Factor-Based Biosensors.ACS synthetic biology · 2025Review
- Sequence-Based Protein Design: A Review of Using Statistical Models to Characterize Coevolutionary Traits for Developing Hybrid Proteins as Genetic Sensors.International journal of molecular sciences · 2024Review
- Rugged fitness landscapes minimize promiscuity in the evolution of transcriptional repressors.Cell systems · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- Advances in ligand-specific biosensing for structurally similar molecules.Cell systems · 2023Review
- Multi-input Drug-Controlled Switches of Mammalian Gene Expression Based on Engineered Nuclear Hormone Receptors.ACS synthetic biology · 2023Article
- Performance Prediction of Fundamental Transcriptional Programs.ACS synthetic biology · 2023Article
- Multi-input drug-controlled switches of mammalian gene expression based on engineered nuclear hormone receptors.bioRxiv : the preprint server for biology · 2023Article
- Deduction of signaling mechanisms from cellular responses to multiple cues.NPJ systems biology and applications · 2022Article
- Synthetic cells in biomedical applications.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2022Review
- Article
- Biological signal processing filters via engineering allosteric transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Coevolutionary methods enable robust design of modular repressors by reestablishing intra-protein interactions.Nature communications · 2021Article
- Transcription Factors as Evolvable Biosensors.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2021Review
- Engineered systems of inducible anti-repressors for the next generation of biological programming.Nature communications · 2020Article
- Majority sensing in synthetic microbial consortia.Nature communications · 2020Article
Corrections and comments
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5 authors.
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Abstract
In prokaryotes, the construction of synthetic, multi-input promoters is constrained by the number of transcription factors that can simultaneously regulate a single promoter. This fundamental engineering constraint is an obstacle to synthetic biologists because it limits the computational capacity of engineered gene circuits. Here, we demonstrate that complex multi-input transcriptional logic gating can be achieved through the use of ligand-inducible chimeric transcription factors assembled from the LacI/GalR family. These modular chimeras each contain a ligand-binding domain and a DNA-binding domain, both of which are chosen from a library of possibilities. When two or more chimeras have the same DNA-binding domain, they independently and simultaneously regulate any promoter containing the appropriate operator site. In this manner, simple transcriptional AND gating is possible through the combination of two chimeras, and multiple-input AND gating is possible with the simultaneous use of three or even four chimeras. Furthermore, we demonstrate that orthogonal DNA-binding domains and their cognate operators allow the coexpression of multiple, orthogonal AND gates. Altogether, this work provides synthetic biologists with novel, ligand-inducible logic gates and greatly expands the possibilities for engineering complex synthetic gene circuits.
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