ArticleThe Journal of biological chemistry2014
Giant virus Megavirus chilensis encodes the biosynthetic pathway for uncommon acetamido sugars.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 25 citations in OpenAlex.
- Surface fibrils on the particles of nucleocytoviruses: A review.Experimental biology and medicine (Maywood, N.J.) · 2023Review
- Virologs, viral mimicry, and virocell metabolism: the expanding scale of cellular functions encoded in the complex genomes of giant viruses.FEMS microbiology reviews · 2023Article
- Analysis of the Genomic Features and Evolutionary History of Pithovirus-Like Isolates Reveals Two Major Divergent Groups of Viruses.Journal of virology · 2023Article
- Assessing the biogeography of marine giant viruses in four oceanic transects.ISME communications · 2023Article
- Assessing the biogeography of marine giant viruses in four oceanic transects.bioRxiv : the preprint server for biology · 2023Article
- The Astounding World of Glycans from Giant Viruses.Chemical reviews · 2022Review
- Giant viruses of themicroLife · 2022Article
- Review
- Giant virus vs amoeba: fight for supremacy.Virology journal · 2019Review
- The rare sugarThe Journal of biological chemistry · 2017Article
- Nothing in Evolution Makes Sense Except in the Light of Genomics: Read-Write Genome Evolution as an Active Biological Process.Biology · 2016Review
- The Autonomous Glycosylation of Large DNA Viruses.International journal of molecular sciences · 2015Review
- The megavirus chilensis Cu,Zn-superoxide dismutase: the first viral structure of a typical cellular copper chaperone-independent hyperstable dimeric enzyme.Journal of virology · 2015Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Giant viruses mimicking microbes, by the sizes of their particles and the heavily glycosylated fibrils surrounding their capsids, infect Acanthamoeba sp., which are ubiquitous unicellular eukaryotes. The glycans on fibrils are produced by virally encoded enzymes, organized in gene clusters. Like Mimivirus, Megavirus glycans are mainly composed of virally synthesized N-acetylglucosamine (GlcNAc). They also contain N-acetylrhamnosamine (RhaNAc), a rare sugar; the enzymes involved in its synthesis are encoded by a gene cluster specific to Megavirus close relatives. We combined activity assays on two enzymes of the pathway with mass spectrometry and NMR studies to characterize their specificities. Mg534 is a 4,6-dehydratase 5-epimerase; its three-dimensional structure suggests that it belongs to a third subfamily of inverting dehydratases. Mg535, next in the pathway, is a bifunctional 3-epimerase 4-reductase. The sequential activity of the two enzymes leads to the formation of UDP-l-RhaNAc. This study is another example of giant viruses performing their glycan synthesis using enzymes different from their cellular counterparts, raising again the question of the origin of these pathways.
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