ArticleJournal of muscle research and cell motility1989
Myosin filaments isolated from skinned amphibian smooth muscle cells are side-polar.
Article in Journal of muscle research and cell motility, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 58 citations in OpenAlex.
- Membrane adhesion junctions regulate airway smooth muscle phenotype and function.Physiological reviews · 2023Review
- Self-assembly of smooth muscle myosin filaments: adaptation of filament length by telokin and Mg·ATP.European biophysics journal : EBJ · 2022Article
- Myosin Crossbridge, Contractile Unit, and the Mechanism of Contraction in Airway Smooth Muscle: A Mechanical Engineer's Perspective.Journal of engineering and science in medical diagnostics and therapy · 2019Review
- Coupling between myosin head conformation and the thick filament backbone structure.Journal of structural biology · 2017Article
- Helical model of smooth muscle myosin filament and the ribbons made of caldesmon: history revisited.European biophysics journal : EBJ · 2016Article
- Velocities of unloaded muscle filaments are not limited by drag forces imposed by myosin cross-bridges.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- The kinetics underlying the velocity of smooth muscle myosin filament sliding on actin filaments in vitro.The Journal of biological chemistry · 2014Article
- Myosin filaments in smooth muscle cells do not have a constant length.The Journal of physiology · 2013Article
- Avian synaptopodin 2 (fesselin) stabilizes myosin filaments and actomyosin in the presence of ATP.Biochemistry · 2013Article
- Caldesmon and the regulation of cytoskeletal functions.Advances in experimental medicine and biology · 2008Review
- Smitin, a novel smooth muscle titin-like protein, interacts with myosin filaments in vivo and in vitro.The Journal of cell biology · 2002Article
- Purification of native myosin filaments from muscle.Biophysical journal · 2001Article
- Assembly of smooth muscle myosin by the 38k protein, a homologue of a subunit of pre-mRNA splicing factor-2.The Journal of cell biology · 2000Article
- Myosin filament structure in vertebrate smooth muscle.The Journal of cell biology · 1996Article
- Mode of caldesmon binding to smooth muscle thin filament: possible projection of the amino-terminal of caldesmon from native thin filament.Biophysical journal · 1995Article
- Calmodulin and the regulation of smooth muscle contraction.Molecular and cellular biochemistry · 1994Review
- Caldesmon binds to smooth muscle myosin and myosin rod and crosslinks thick filaments to actin filaments.Journal of muscle research and cell motility · 1992Article
- The molecular anatomy of caldesmon.The Biochemical journal · 1991Review
- Force response to rapid length change during contraction and rigor in skinned smooth muscle of guinea-pig taenia coli.The Journal of physiology · 1991Article
- A nucleation--elongation mechanism for the self-assembly of side polar sheets of smooth muscle myosin.The EMBO journal · 1991Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The structure of myosin filaments isolated from skinned toad stomach smooth muscle cells has been examined by electron microscopy as a step toward identifying the in vivo structure. When negatively stained following exposure to relaxing conditions, the filaments exhibited a continuous 14-nm axial repeat of crossbridge projections with no central bare zone. The filaments thus differed from the bipolar filaments found in striated muscle and displayed instead features resembling side-polar and mixed-polarity filament models. By rotation of isolated filaments around their longitudinal axes it was found that cross bridges occurred only along two sides of the filament, an arrangement consistent with the side-polar but not the mixed-polarity model. The polarity is thus similar to that proposed for ribbons (Small & Squire, J. molec. Biol. 67, (1972) 17-149) and for synthetic smooth muscle myosin filaments (Craig and Megerman, J. Cell Biol. 75, (1977) 990-996); their appearance in cross-section, however, shows that these structures are filaments (i.e. with two axes of similar dimensions) and not broad ribbons. As the filaments were derived directly from skinned cells which contracted and relaxed in response to physiological levels of MgATP and Ca2+ at rates comparable to those of native, isolated cells, this unusual arrangement of cross bridges appears to be an effective, functional form of myosin in the contractile apparatus. Side-polar filaments therefore merit consideration as plausible candidates for the native organization of myosin in vertebrate smooth muscle cells.
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