ArticleInvestigative ophthalmology & visual science2014
Bis(zinc-dipicolylamine), Zn-DPA, a new marker for apoptosis.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 30 citations in OpenAlex.
- Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling.Cellular & molecular biology letters · 2023Article
- Diabetes mellitus associated neurovascular lesions in the retina and brain: A review.Frontiers in ophthalmology · 2022Review
- Retinal Dysfunction in Alzheimer's Disease and Implications for Biomarkers.Biomolecules · 2021Review
- Non-invasive in vivo fluorescence imaging of apoptotic retinal photoreceptors.Scientific reports · 2019Article
- Imaging and therapeutic applications of zinc(ii)-dipicolylamine molecular probes for anionic biomembranes.Chemical communications (Cambridge, England) · 2016Review
- Binding of bacteria to magnetic nanoparticles in suspension: Experiments and kinetic models.Biotechnology progressArticle
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo characterize the labeling of apoptotic cells with a molecular probe of bis(zinc(II)-dipicolylamine) (Zn-DPA) conjugated with a fluorescent reporter in a rat model of retinal ganglion cell (RGC) degeneration induced by N-methyl-D-aspartate (NMDA).
methodsAdult Wistar rats were given unilateral intravitreal injections of 3 μL 40 mM neutralized NMDA and euthanized at 1, 2, 4, 24, and 48 hours. One hour before euthanasia, 3 μL Zn-DPA conjugated with fluorescein (Zn-DPA 480) was intravitreally injected. Prelabeling of RGC with retrograde fluorogold (FG), TUNEL, and immunohistochemistry with III β-tubulin and vimentin were performed.
resultsFluorescence labeling of Zn-DPA 480 was observed in the retinas from 1 hour up to 24 hours after NMDA injection, whereas the labeling was reduced at 48 hours postinjection. At both 4 and 24 hours postinjection, most Zn-DPA 480-positive cells in the RGC layer were labeled by FG and III β-tubulin. The number of TUNEL-positive cells increased from 4 to 24 hours. At 24 hours, 95.7% of Zn-DPA 480-positive cells were TUNEL positive, whereas 95.1% of TUNEL-positive cells were Zn-DPA 480 positive. The numbers of Zn-DPA 480-positive cells at 1 and 2 hours after NMDA injection were significantly higher than TUNEL.
conclusionsOur findings demonstrate that intravitreal injection of fluorescent Zn-DPA 480 labels retinal neurons undergoing apoptosis and that recognition of exposed phosphatidylserine appears earlier than detection of DNA fragmentation, indicating the potential of Zn-DPA as an imaging probe for tracking degenerating retinal neurons.
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